Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Honda, S.; Toraya, T.; Fukui, S.
    In situ reactivation of glycerol-inactivated coenzyme B12-dependent enzymes, glycerol dehydratase and diol dehydratase (1980), J. Bacteriol., 143, 1458-1465.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 glycerol inactivation of the enzyme in situ and in vitro during catalysis, glycerol inactivated enzyme in situ is reactivated by ATP and Mn2+ in the presence of free adenosylcobalamin. CTP and GTP are able to replace ATP partially. Mn2+ can be fully replaced by Mg2+ Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 0.2
-
 1,2-propanediol enzyme in situ, toluene-treated cells Klebsiella pneumoniae
4.2.1.30 0.36
-
 1,2-propanediol enzyme in vitro Klebsiella pneumoniae
4.2.1.30 0.53
-
 glycerol enzyme in situ, toluene-treated cells Klebsiella pneumoniae
4.2.1.30 0.54
-
 glycerol enzyme in vitro Klebsiella pneumoniae
4.2.1.30 1.08
-
 ethanediol enzyme in situ, toluene-treated cells Klebsiella pneumoniae
4.2.1.30 1.56
-
 ethanediol enzyme in vitro Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.30 glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
 available as Enterobacter aerogenes ATCC 25955
-
4.2.1.30 Klebsiella pneumoniae
-
 ATCC 25955
-
4.2.1.30 Klebsiella pneumoniae PZH 572 Warsaw
-
 available as Enterobacter aerogenes ATCC 25955
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 additional information
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-ethanediol
-
 Klebsiella pneumoniae acetaldehyde + H2O
-
 ?
4.2.1.30 1,2-ethanediol
-
 Klebsiella pneumoniae PZH 572 Warsaw acetaldehyde + H2O
-
 ?
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae PZH 572 Warsaw propionaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae PZH 572 Warsaw ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae