Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Johnson, B.C.; Stroinski, A.; Schneider, Z.
    Glycerol dehydratase from Aerobacter aerogenes (1975), Methods Enzymol., 42C, 315-323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 additional information highly specific antagonistic effect of sodium versus potassium Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 EDTA causes inactivation by dissociation of the apoenzyme into its subunits. Inhibition is reversed by dialysis, Mg2+, glycerol or by addition of excess of either subunit Klebsiella pneumoniae
4.2.1.30 N-ethylmaleimide 1.0 mM blocks enzymatic reaction Klebsiella pneumoniae
4.2.1.30 p-chloromercuribenzoate 1.0 mM block enzymatic reaction Klebsiella pneumoniae
4.2.1.30 salicylic acid causes inactivation by dissociation of the apoenzyme into its subunits Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 1.25
-
glycerol in the presence of 0.1 M potassium phosphate buffer Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 K+ required Klebsiella pneumoniae
4.2.1.30 K+ in the absence of other monovalent ions optimum K+ concentration: 0.08-0.15 M Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 22000
-
gel filtration, subunit A, 20 mM potassium phosphate, pH 7.7, subunit A dissociates into sub-subunits of 11000 Da Klebsiella pneumoniae
4.2.1.30 90000
-
gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits Klebsiella pneumoniae
4.2.1.30 188000
-
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae
4.2.1.30 189000
-
gel filtration in the presence of 50 mM ethanolamine-HCl buffer, pH 8.6, subunit B Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
?
-
?
4.2.1.30 glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
available as Enterobacter aerogenes ATCC 25955
-
4.2.1.30 Klebsiella pneumoniae
-
ATCC 25955
-
4.2.1.30 Klebsiella pneumoniae PZH 572 Warsaw
-
available as Enterobacter aerogenes ATCC 25955
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.30 holoenzyme can be protected from oxygen by addition of 50 mM K2SO3 Klebsiella pneumoniae

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 apoenzyme, AB Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 228
-
apoenzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-propanediol propane-1,2-diol Klebsiella pneumoniae propionaldehyde + H2O
-
?
4.2.1.30 1,2-propanediol propane-1,2-diol Klebsiella pneumoniae PZH 572 Warsaw propionaldehyde + H2O
-
?
4.2.1.30 ethylene glycol
-
Klebsiella pneumoniae acetaldehyde + H2O
-
?
4.2.1.30 ethylene glycol
-
Klebsiella pneumoniae PZH 572 Warsaw acetaldehyde + H2O
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
Klebsiella pneumoniae ?
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 dimer the dissociation and association of the apoenzyme into subunits is influenced by pH, monovalent cations and glycerol Klebsiella pneumoniae
4.2.1.30 More
-
Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.30 additional information
-
-
Klebsiella pneumoniae
4.2.1.30 46
-
4 min, 50% destruction of the apoenzyme Klebsiella pneumoniae
4.2.1.30 70
-
inactivation of subunit A at 70°C is reversible Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.30 8.6
-
-
Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.30 8 9 enzyme activity between Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 5,6-dimethylbenzimidazolylcobamide 5'-phosphate i.e. DBCC, the inactive enzyme complex with hydroxocobalamin can be converted to the active holoenzyme by replacement of hydroxocobalamin with DBCC in the presence of MG2+ and SO32- Klebsiella pneumoniae