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Literature summary extracted from

  • Ueda, Y.; Yumoto, N.; Tokushige, M.; Fukui, K.; Ohya-Nishiguchi, H.
    Purification and characterization of two types of fumarase from Escherichia coli (1991), J. Biochem., 109, 728-733.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.2 (NH4)2SO4, protects enzyme form FUMA from inactivation at 4°C Escherichia coli
4.2.1.2 10% glycerol, 0.1 M KCl, 5 mM L-malate or 30% ethyleneglycol, partially protects enzyme form FUMA from inactivation at 4°C Escherichia coli
4.2.1.2 enzyme form FUMA, oxidation and the concomitant release of iron inactivates the enzyme in a reversible manner Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 ammonium persulfate
-
 Escherichia coli
4.2.1.2 pyromellilate
-
 Escherichia coli
4.2.1.2 S-2,3-dicarboxyaziridine enzyme form FUMC is inhibited, enzyme form FUMA is not inhibited Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.049
-
 L-malate enzyme form FUMA Escherichia coli
4.2.1.2 0.05
-
 L-malate enzyme form FUMC Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.2 Iron one Fe-S-dependent enzyme form and one Fe-S-independent enzyme form Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 60000
-
 2 * 60000, enzyme form FUMA, SDS-PAGE Escherichia coli
4.2.1.2 120000
-
 enzyme form FUMA, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Escherichia coli
-
 2 types of enzyme: Fe-independent FUMC and Fe-dependent FUMA
-
4.2.1.2 Escherichia coli
-
 W
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2 FUMA and FUMC Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 340
-
 FUMA Escherichia coli
4.2.1.2 730
-
 FUMC Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Escherichia coli fumarate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 dimer 2 * 60000, enzyme form FUMA, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 49
-
 5 min, 50% inhibition, enzyme form FUMA Escherichia coli
4.2.1.2 51
-
 5 min, 50% inhibition, enzyme form FUMA Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8
-
 enzyme form FUMC Escherichia coli
4.2.1.2 8.5
-
 enzyme form FUMA Escherichia coli