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Literature summary extracted from

  • Waterson, R.M.; Hill, R.L.
    Enoyl coenzyme A hydratase (crotonase). Catalytic properties of crotonase and its possible regulatory role in fatty acid oxidation (1972), J. Biol. Chem., 247, 5258-5265.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.17 acetoacetyl-CoA enolate form of acetoacetyl-CoA acts as a competitive inhibitor. 6 molecules of inhibitor are bound per molecule of enzyme Bos taurus
4.2.1.17 decenoyl-CoA
-
 Bos taurus
4.2.1.17 hexadecenoyl-CoA
-
 Bos taurus
4.2.1.17 hexenoyl-CoA
-
 Bos taurus
4.2.1.17 additional information no inhibition: ethyl acetoacetate, acetoacetate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.17 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Bos taurus
-
 The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.17 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 (2E)-octenoyl-CoA + H2O 36% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?
4.2.1.17 crotonyl-CoA + H2O best substrate. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?
4.2.1.17 decenoyl-CoA + H2O 17% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?
4.2.1.17 dodecenoyl-CoA + H2O 7% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?
4.2.1.17 hexadecenoyl-CoA + H2O 1% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?
4.2.1.17 hexenoyl-CoA + H2O 67% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?
4.2.1.17 tetradecenoyl-CoA + H2O 2% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.17 hexamer
-
 Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 crotonase the classification is ambiguous because the stereochemistry is not exactly determined Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.17 5667
-
 crotonyl-CoA pH 7.5, 25°C Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.17 0.00024
-
 hexenoyl-CoA pH 7.5, 25°C Bos taurus
4.2.1.17 0.00028
-
 octenoyl-CoA pH 7.5, 25°C Bos taurus
4.2.1.17 0.0003
-
 decenoyl-CoA pH 7.5, 25°C Bos taurus
4.2.1.17 0.0004
-
 dodecenoyl-CoA pH 7.5, 25°C Bos taurus
4.2.1.17 0.00042
-
 tetradecenoyl-CoA pH 7.5, 25°C Bos taurus
4.2.1.17 0.0005
-
 hexadecenoyl-CoA pH 7.5, 25°C Bos taurus
4.2.1.17 0.0016
-
 acetoacetyl-CoA pH 7.5, 25°C Bos taurus