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Literature summary extracted from

  • Nakagawa, T.; Nagayama, F.
    Enzymatic properties of enolase from fish muscle (1991), Comp. Biochem. Physiol. B, 98, 355-359.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Cyprinus carpio
4.2.1.11 phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Oryctolagus cuniculus
4.2.1.11 phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Pagrus major
4.2.1.11 phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Scomber japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.038
-
 2-phospho-D-glycerate
-
 Scomber japonicus
4.2.1.11 0.05
-
 2-phospho-D-glycerate
-
 Pagrus major
4.2.1.11 0.061
-
 2-phospho-D-glycerate
-
 Oryctolagus cuniculus
4.2.1.11 0.069
-
 2-phospho-D-glycerate
-
 Cyprinus carpio
4.2.1.11 0.11
-
 phosphoenolpyruvate
-
 Scomber japonicus
4.2.1.11 0.2
-
 phosphoenolpyruvate
-
 Pagrus major
4.2.1.11 0.2
-
 phosphoenolpyruvate
-
 Cyprinus carpio
4.2.1.11 0.25
-
 phosphoenolpyruvate
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ Km: 0.7 mM Pagrus major
4.2.1.11 Mg2+ Km: 0.67 mM Cyprinus carpio
4.2.1.11 Mg2+ Km: 0.73 mM Scomber japonicus
4.2.1.11 Mg2+ Km: 0.69 mM Oryctolagus cuniculus
4.2.1.11 Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Oryctolagus cuniculus
4.2.1.11 Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Pagrus major
4.2.1.11 Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Scomber japonicus
4.2.1.11 Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Cyprinus carpio
4.2.1.11 Mn2+ can partially replace Mg2+ in activation Oryctolagus cuniculus
4.2.1.11 Mn2+ can partially replace Mg2+ in activation Pagrus major
4.2.1.11 Mn2+ can partially replace Mg2+ in activation Scomber japonicus
4.2.1.11 Mn2+ can partially replace Mg2+ in activation Cyprinus carpio
4.2.1.11 Zn2+ can partially replace Mg2+ in activation Oryctolagus cuniculus
4.2.1.11 Zn2+ can partially replace Mg2+ in activation Pagrus major
4.2.1.11 Zn2+ can partially replace Mg2+ in activation Scomber japonicus
4.2.1.11 Zn2+ can partially replace Mg2+ in activation Cyprinus carpio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 48000
-
 2 * 48000, SDS-PAGE Oryctolagus cuniculus
4.2.1.11 50000
-
 2 * 50000, SDS-PAGE Scomber japonicus
4.2.1.11 51000
-
 2 * 51000, SDS-PAGE Cyprinus carpio
4.2.1.11 52000
-
 2 * 52000, SDS-PAGE Pagrus major
4.2.1.11 92000
-
 gel filtration Oryctolagus cuniculus
4.2.1.11 93000
-
 gel filtration Scomber japonicus
4.2.1.11 94000
-
 gel filtration Cyprinus carpio
4.2.1.11 95500
-
 gel filtration Pagrus major

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Cyprinus carpio
-
-
-
4.2.1.11 Oryctolagus cuniculus
-
-
-
4.2.1.11 Pagrus major
-
-
-
4.2.1.11 Scomber japonicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 muscle
-
 Oryctolagus cuniculus
-
4.2.1.11 muscle
-
 Pagrus major
-
4.2.1.11 muscle
-
 Scomber japonicus
-
4.2.1.11 muscle
-
 Cyprinus carpio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 56.1
-
-
 Scomber japonicus
4.2.1.11 61.7
-
-
 Scomber japonicus
4.2.1.11 76.2
-
-
 Pagrus major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Oryctolagus cuniculus phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Pagrus major phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Scomber japonicus phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Cyprinus carpio phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Oryctolagus cuniculus phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Pagrus major phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Scomber japonicus phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Cyprinus carpio phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 phosphoenolpyruvate
-
 Oryctolagus cuniculus 2-phospho-D-glycerate
-
 r
4.2.1.11 phosphoenolpyruvate
-
 Pagrus major 2-phospho-D-glycerate
-
 r
4.2.1.11 phosphoenolpyruvate
-
 Scomber japonicus 2-phospho-D-glycerate
-
 r
4.2.1.11 phosphoenolpyruvate
-
 Cyprinus carpio 2-phospho-D-glycerate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 50000, SDS-PAGE Scomber japonicus
4.2.1.11 dimer 2 * 51000, SDS-PAGE Cyprinus carpio
4.2.1.11 dimer 2 * 48000, SDS-PAGE Oryctolagus cuniculus
4.2.1.11 dimer 2 * 52000, SDS-PAGE Pagrus major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 6.6
-
 formation of 2-phosphoglycerate Scomber japonicus
4.2.1.11 6.6
-
 formation of 2-phosphoglycerate Cyprinus carpio
4.2.1.11 6.9
-
 formation of phosphoenolpyruvate Oryctolagus cuniculus
4.2.1.11 6.9
-
 formation of phosphoenolpyruvate Cyprinus carpio
4.2.1.11 7
-
-
 Oryctolagus cuniculus
4.2.1.11 7.1
-
 formation of 2-phosphoglycerate Pagrus major
4.2.1.11 7.1
-
 formation of phosphoenolpyruvate Scomber japonicus
4.2.1.11 7.3
-
 formation of phosphoenolpyruvate Pagrus major