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Literature summary extracted from

  • Rider, C.C.; Taylor, C.B.
    Enolase isoenzymes in rat tissues. Electrophoretic, chromatographic, immunological and kinetic properties (1974), Biochim. Biophys. Acta, 365, 285-300.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.12
-
 2-phospho-D-glycerate muscle enzyme Rattus norvegicus
4.2.1.11 0.14
-
 2-phospho-D-glycerate liver enzyme Rattus norvegicus
4.2.1.11 0.31
-
 phosphoenolpyruvate liver enzyme Rattus norvegicus
4.2.1.11 0.37
-
 phosphoenolpyruvate muscle enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 49000
-
 2 * 49000, muscle enzyme, SDS-PAGE Rattus norvegicus
4.2.1.11 53000
-
 2 * 53000, liver enzyme, SDS-PAGE Rattus norvegicus
4.2.1.11 91000
-
 liver enzyme, gel filtration Rattus norvegicus
4.2.1.11 93000
-
 muscle enzyme, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 liver enzyme Rattus norvegicus
4.2.1.11 muscle enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 adipose tissue enolase I Rattus norvegicus
-
4.2.1.11 blood enolase I Rattus norvegicus
-
4.2.1.11 heart enolase I and III Rattus norvegicus
-
4.2.1.11 kidney enolase I Rattus norvegicus
-
4.2.1.11 liver enolase I Rattus norvegicus
-
4.2.1.11 muscle contains only enolase III Rattus norvegicus
-
4.2.1.11 spleen enolase I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 87
-
 muscle enolase Rattus norvegicus
4.2.1.11 120
-
 muscle enolase Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
4.2.1.11 4°C, stable for several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Rattus norvegicus phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Rattus norvegicus phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 phosphoenolpyruvate
-
 Rattus norvegicus 2-phospho-D-glycerate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 53000, liver enzyme, SDS-PAGE Rattus norvegicus
4.2.1.11 dimer 2 * 49000, muscle enzyme, SDS-PAGE Rattus norvegicus