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Literature summary extracted from

  • Oh, S.K.; Brewer, J.M.
    Purification and properties of enolase from swine kidney (1973), Arch. Biochem. Biophys., 157, 491-499.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.11 Mg2+, 20% sucrose or glycerol stabilize enzyme in dilute solutions Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 2-phosphoglycerate substrate inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 additional information
-
 additional information
-
 Sus scrofa
4.2.1.11 0.05
-
 2-phospho-D-glycerate
-
 Sus scrofa
4.2.1.11 0.11
-
 phosphoenolpyruvate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 KCl required for maximal activity Sus scrofa
4.2.1.11 Mg2+ Km: 0.43 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 52000
-
 2 * 52000, SDS-PAGE Sus scrofa
4.2.1.11 88000
-
 sedimentation velocity measurement Sus scrofa
4.2.1.11 110000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 kidney
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
4.2.1.11 4°C, 1 mg/ml or more, 0.1 M imidazole HCl, pH 7.0 and 2 mM MgCl2, stable for at least 2 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Sus scrofa phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Sus scrofa phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 phosphoenolpyruvate
-
 Sus scrofa 2-phospho-D-glycerate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 52000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 6.8
-
-
 Sus scrofa