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Literature summary extracted from

  • Elling, L.; Ritter, J.E.; Verseck, S.
    Expression, purification and characterization of recombinant phosphomannomutase and GDP-alpha-D-mannose pyrophosphorylase from Salmonella enterica, group B, for the synthesis of GDP-alpha-D-mannose from D-mannose (1996), Glycobiology, 6, 591-597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 overexpression in Escherichia coli Salmonella enterica
5.4.2.8 overexpression in Escherichia coli BL21 Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 GDP-mannose competitive vs. GTP, uncompetitive vs. mannose 1-phosphate Salmonella enterica
2.7.7.13 GDP-mannose
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.01
-
 alpha-D-mannose 1-phosphate
-
 Salmonella enterica
2.7.7.13 0.2
-
 GTP
-
 Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 54045
-
 2 * 54045, recombinant enzyme, deduced from nucleotide sequence Salmonella enterica
2.7.7.13 55000
-
 2 * 55000, recombinant enzyme, SDS-PAGE Salmonella enterica
2.7.7.13 108000
-
 recombinant enzyme, gel filtration Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
 GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Arthrobacter sp.
-
 GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Salmonella enterica
-
 GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Arthrobacter sp.
-
-
-
2.7.7.13 Salmonella enterica
-
 group B
-
2.7.7.13 Sus scrofa
-
-
-
5.4.2.8 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 recombinant enzyme, Q-Sepharose FF, Phenyl-Sepharose, diafiltration, Sephadex G 75, Sephadex G 200, ammonium sulfate Salmonella enterica
5.4.2.8 partial Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 2.75
-
 recombinant enzyme Salmonella enterica
5.4.2.8 0.26
-
-
 Salmonella enterica

Storage Stability

EC Number Storage Stability Organism
2.7.7.13 4°C, 25% glycerol, 47 d, 25% loss of activity Salmonella enterica
2.7.7.13 4°C, 3 M ammonium sulfate, 47 d, 40% loss of activity, 122 d, 50% loss of activity Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Sus scrofa GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Arthrobacter sp. GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Salmonella enterica GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Salmonella enterica diphosphate + GDP-mannose
-
 ?
5.4.2.8 D-Mannose 1-phosphate
-
 Salmonella enterica D-Mannose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 dimer 2 * 55000, recombinant enzyme, SDS-PAGE Salmonella enterica
2.7.7.13 dimer 2 * 54045, recombinant enzyme, deduced from nucleotide sequence Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 GDP-alpha-D-mannose pyrophosphorylase
-
 Salmonella enterica
2.7.7.13 RfbM enzyme is encoded by the rfbM gene Salmonella enterica
2.7.7.13 RfbM
-
 Salmonella enterica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.13 0.015
-
 GDP-mannose competitive vs. GTP Salmonella enterica
2.7.7.13 0.12
-
 GDP-mannose uncompetitive vs. mannose 1-phosphate Salmonella enterica