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Literature summary extracted from

  • Jones, G.L.; Shaw, D.C.
    Purification, properties, partial sequence and evolutionary relationships of marsupial erythrocyte carbonic anhydrase (1982), Biochim. Biophys. Acta, 709, 284-303.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.1 dialysis against pyridine dicarboxylate, at 15°C, 98% loss of activity after 24 h Macropus eugenii desmarest

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 dithiothreitol
-
 Macropus eugenii desmarest
4.2.1.1 p-hydroxymercuribenzoate
-
 Macropus eugenii desmarest
4.2.1.1 PCMB reversible with 5 mM dithiothreitol Macropus eugenii desmarest

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 2.67
-
 CO2
-
 Macropus eugenii desmarest

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Co2+ when added back to the apoenzyme in a 1:1 molar ratio or greater, the activity immediately returns to control level Macropus eugenii desmarest
4.2.1.1 Ni2+ slight activation of the apoenzyme Macropus eugenii desmarest
4.2.1.1 Zinc Zn2+, when added back to the apoenzyme in a 1:1 molar ratio or greater, the activity immediately returns to control level Macropus eugenii desmarest

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 28200
-
 1 * 28200, carbonic anhydrase A, SDS-PAGE Macropus eugenii desmarest
4.2.1.1 30700
-
 gel filtration Macropus eugenii desmarest

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Macropus eugenii desmarest
-
 tammar wallaby
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Macropus eugenii desmarest

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 erythrocyte
-
 Macropus eugenii desmarest
-

Storage Stability

EC Number Storage Stability Organism
4.2.1.1 4°C, stable for up to 1 year Macropus eugenii desmarest

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Macropus eugenii desmarest H2CO3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 monomer 1 * 28200, carbonic anhydrase A, SDS-PAGE Macropus eugenii desmarest

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 55
-
 t1/2: more than 120 min Macropus eugenii desmarest