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Literature summary extracted from

  • Murata, T.
    Purification and some properties of phosphomannomutase from corms of Amorphophallus konjac C. Koch (1976), Plant Cell Physiol., 17, 1099-1109.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.8 alpha-D-glucose 1,6-diphosphate required Amorphophallus konjac

General Stability

EC Number General Stability Organism
5.4.2.8 Mg2+ stabilizes Amorphophallus konjac

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.8 Be2+ in histidine buffer Amorphophallus konjac
5.4.2.8 Co2+ weak Amorphophallus konjac
5.4.2.8 Cu2+ in Tris buffer, no inhibition in histidine buffer Amorphophallus konjac
5.4.2.8 mannose 1-phosphate at high concentrations, competitive with respect to glucose-1,6-diphosphate, Ki: 1.2 mM Amorphophallus konjac
5.4.2.8 Ni2+ weak Amorphophallus konjac
5.4.2.8 Zn2+ in Tris buffer, no inhibition in histidine buffer Amorphophallus konjac

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.8 0.2
-
 mannose 1-phosphate
-
 Amorphophallus konjac

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.8 Co2+ 72% of the activation relative to Mg2+, maximal activity at 0.5 mM Amorphophallus konjac
5.4.2.8 Mg2+ required Amorphophallus konjac
5.4.2.8 Mg2+ maximal activity at 1 mM Amorphophallus konjac
5.4.2.8 Ni2+ 36% of the activation relative to Mg2+, maximal activity at 0.5 mM Amorphophallus konjac

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.8 62000
-
 gel filtration Amorphophallus konjac

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.8 Amorphophallus konjac
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.8
-
 Amorphophallus konjac

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.8 corm
-
 Amorphophallus konjac
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.8 6.105
-
-
 Amorphophallus konjac

Storage Stability

EC Number Storage Stability Organism
5.4.2.8 -20°C, stable for at least 2 months Amorphophallus konjac

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.8 D-Mannose 1-phosphate
-
 Amorphophallus konjac D-Mannose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.8 40
-
-
 Amorphophallus konjac

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.8 38
-
 50% loss of activity within a few min, completely protected from heat inactivation by 1 mM mannose 1-phosphate, 0.01 mM glucose 1,6-diphosphate or 1 mM fructose 1,6-diphosphate. In presence of 1 mM Mg2+: 50% loss of activity after 17 min Amorphophallus konjac

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.8 6.5 7
-
 Amorphophallus konjac

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.2.8 7.5
-
 maximal stability Amorphophallus konjac