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Literature summary extracted from

  • Aisaka, K.; Igarashi, A.; Yamaguchi, K.; Uwajima, T.
    Purification, crystallization and characterization of N-acetylneuraminate lyase from Escherichia coli (1991), Biochem. J., 276, 541-546.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.3
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 Cu2+ 7% residual activity at 1 mM Escherichia coli
4.1.3.3 diethyldicarbonate 28% residual activity at 1 mM Escherichia coli
4.1.3.3 dihydroxyacetone 2% residual activity at 10 mM Escherichia coli
4.1.3.3 DL-glyceraldehyde 0% residual activity at 10 mM Escherichia coli
4.1.3.3 Fe2+ 78% residual activity at 1 mM Escherichia coli
4.1.3.3 N-bromosuccinimide 0% residual activity at 0.1 mM, 36% residual activity at 0.01 mM Escherichia coli
4.1.3.3 NaBH4
-
 Escherichia coli
4.1.3.3 p-chloromercuribenzoate 0% residual activity at 0.01 mM, 44% residual activity at 0.001 mM Escherichia coli
4.1.3.3 pyruvate 30% residual activity at 10 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 3.3
-
 N-acetylneuraminic acid
-
 Escherichia coli
4.1.3.3 3.3
-
 N-glycolylneuraminic acid
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 35000
-
 3 * 35000, SDS-PAGE Escherichia coli
4.1.3.3 105000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Escherichia coli
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.3.3 sensitive to illumination in presence of Rose Bengal Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.3 35.5
-
 N-acetylneuraminic acid Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-Glycolylneuraminic acid
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.3 trimer 3 * 35000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.3 80
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.3 additional information
-
 pyruvate protects against heat inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 6.5 7 elimination Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.3.3 6 7 at 75°C stable for more than 15 min Escherichia coli