Literature summary extracted from
Belocopitow, E.; Marechal, L.R.
Metabolism of trehalose in Euglena gracilis. Partial purification and some properties of phosphoglucomutase acting on beta-glucose 1-phosphate (1974), Eur. J. Biochem., 46, 631-637.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
5.4.2.6 |
arsenate |
increasemM s activity |
Euglena gracilis |
|
5.4.2.6 |
beta-D-Glucose 1,6-diphosphate |
required |
Euglena gracilis |
|
5.4.2.6 |
beta-D-Glucose 1,6-diphosphate |
Km: 0.0005 mM |
Euglena gracilis |
|
General Stability
EC Number |
General Stability |
Organism |
---|
5.4.2.6 |
after 12 h in 0.02 M imidazole buffer, at pH 7.0, totally inactivated both at 0°C and at -20°C. Glycylglycine, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and phosphate at the same conditions are able to preserve most of the enzyme activity. Cacodylate, ethylmorpholidate, Tris-maleate and glycerophosphate have an intermediate action |
Euglena gracilis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.4.2.6 |
KF |
10 mM: 75% inhibition, 17 mM: 100% inhibition |
Euglena gracilis |
|
5.4.2.6 |
phosphate |
10 mM, 20% inhibition |
Euglena gracilis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.4.2.6 |
Co2+ |
divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 0.4 mM Co2+ |
Euglena gracilis |
|
5.4.2.6 |
Mg2+ |
divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 1-3 mM Mg2+ |
Euglena gracilis |
|
5.4.2.6 |
Mn2+ |
divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 0.4 mM Mn2+ |
Euglena gracilis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.4.2.6 |
27000 |
- |
gel filtration |
Euglena gracilis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.4.2.6 |
beta-D-Glucose 1-phosphate |
Euglena gracilis |
the enzyme together with a trehalose phosphorylase would constitute a new catabolic pathway for trehalose |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.2.6 |
Euglena gracilis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.2.6 |
partial |
Euglena gracilis |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
5.4.2.6 |
4.463 |
- |
- |
Euglena gracilis |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.4.2.6 |
-70°C, 0.02 M phosphate buffer, pH 7.0, stable for at least 6 months |
Euglena gracilis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.2.6 |
beta-D-Glucose 1-phosphate |
r |
Euglena gracilis |
beta-D-Glucose 6-phosphate |
- |
? |
|
5.4.2.6 |
beta-D-Glucose 1-phosphate |
the enzyme together with a trehalose phosphorylase would constitute a new catabolic pathway for trehalose |
Euglena gracilis |
? |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.4.2.6 |
7 |
- |
- |
Euglena gracilis |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
5.4.2.6 |
6 |
8.2 |
6.0: about 35% of maximal activity, pH 8.2: about 15% of maximal activity |
Euglena gracilis |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
5.4.2.6 |
6 |
- |
0°C: about 70% loss of activity after 2 days, -20°C: about 30% loss of activity after 2 days, -70°C: no loss of activity after 2 days |
Euglena gracilis |