EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.27 | Ba2+ | - |
Ruta graveolens | |
4.1.3.27 | Co2+ | 10 mM, 67% inhibition of enzyme from cell line R-15, 33% inhibition of enzyme from cell line R-20 | Ruta graveolens | |
4.1.3.27 | Cu2+ | - |
Ruta graveolens | |
4.1.3.27 | Hg2+ | 1 mM HgCl2, 94.1% inhibition | Ruta graveolens | |
4.1.3.27 | L-Trp | competitive with respect to chorismate | Ruta graveolens | |
4.1.3.27 | MnCl2 | - |
Ruta graveolens | |
4.1.3.27 | Pb2+ | - |
Ruta graveolens | |
4.1.3.27 | PCMB | 1 mM, 88.6% inhibition | Ruta graveolens | |
4.1.3.27 | Zn2+ | - |
Ruta graveolens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.27 | 0.18 | - |
Gln | cell line R-15 | Ruta graveolens | |
4.1.3.27 | 0.22 | - |
chorismate | cell line R-20 | Ruta graveolens | |
4.1.3.27 | 0.25 | - |
Gln | cell line R-20 | Ruta graveolens | |
4.1.3.27 | 0.28 | - |
chorismate | cell line R-15 | Ruta graveolens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.27 | Co2+ | at 1-5 mM | Ruta graveolens | |
4.1.3.27 | Fe2+ | 1 mM, can partially replace Mg2+ in activation | Ruta graveolens | |
4.1.3.27 | Mg2+ | - |
Ruta graveolens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 220000 | - |
gel filtration | Ruta graveolens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.27 | Ruta graveolens | - |
a high rutacridone producing cell line R-20 and low rutacrinone producing cell line R-15 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.27 | partial, from a high rutacridone producing cell line R-20 and from a low rutacrinone producing cell line R-15 | Ruta graveolens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | additional information | - |
- |
Ruta graveolens |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.3.27 | -20°C, unstable | Ruta graveolens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.27 | chorismate + L-Gln | - |
Ruta graveolens | anthranilate + pyruvate + L-glutamate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 37 | 40 | - |
Ruta graveolens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 25 | 45 | 25°C: about 70% of maximal activity, 45°C: about 85% of maximal activity | Ruta graveolens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 35 | - |
5 min, about 25% loss of activity | Ruta graveolens |
4.1.3.27 | 40 | - |
5 min, about 50% loss of activity | Ruta graveolens |
4.1.3.27 | 45 | - |
5 min, about 80% loss of activity | Ruta graveolens |
4.1.3.27 | 50 | - |
5 min, about 90% loss of activity | Ruta graveolens |