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Literature summary extracted from

  • Shiratsuchi, A.; Sato, S.
    Characterization of Bacillus caldotenax anthranilate synthase I produced in Escherichia coli and identification of its essential arginine residue by site-directed mutagenesis (1992), J. Biochem., 112, 714-718.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.27 R321H Km-value is increased 3fold to 4fold compared to the value for the wild-type enzyme, little effect on turnover number [Bacillus] caldotenax
4.1.3.27 R358H Km-value is increased 3fold to 4fold compared to the value for the wild-type enzyme, little effect on turnover number [Bacillus] caldotenax
4.1.3.27 R429H similar Km value to the wild-type enzyme and a slightly larger turnover number [Bacillus] caldotenax
4.1.3.27 R452H less than 0.05% of the activity of the wild-type enzyme [Bacillus] caldotenax

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-Trp
-
 [Bacillus] caldotenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.038
-
 chorismate at pH 9 and 37°C, wild-type enzyme [Bacillus] caldotenax
4.1.3.27 0.042
-
 chorismate mutant enzyme R429H [Bacillus] caldotenax
4.1.3.27 0.14
-
 chorismate mutant enzyme R358H and R416H [Bacillus] caldotenax
4.1.3.27 0.15
-
 chorismate mutant enzyme R321H [Bacillus] caldotenax
4.1.3.27 46
-
 NH4+ at pH 9 and 37°C [Bacillus] caldotenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+ required [Bacillus] caldotenax
4.1.3.27 Mg2+ maximal activity at 10 mM [Bacillus] caldotenax

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 [Bacillus] caldotenax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 anthranilate synthase I, from a plasmid bearing Escherichia coli [Bacillus] caldotenax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 additional information
-
-
 [Bacillus] caldotenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 [Bacillus] caldotenax anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+ anthranilate synthase I [Bacillus] caldotenax ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.27 60
-
-
 [Bacillus] caldotenax

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.27 383
-
 chorismate at pH 9 and 37°C, with NH4+ as cosubstrate [Bacillus] caldotenax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 9
-
-
 [Bacillus] caldotenax

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.27 6.5 9 pH 6.5: about 20% of maximal activity, pH 9.0: optimum [Bacillus] caldotenax