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Literature summary extracted from

  • Widholm, J.M.
    Anthranilate synthetase from 5-methyltryptophan-susceptible and -resistant cultured Daucus carota cells (1972), Biochim. Biophys. Acta, 279, 48-57.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 Cu2+
-
 Daucus carota
4.1.3.27 DL-4-methyltryptophan
-
 Daucus carota
4.1.3.27 DL-5-fluorotryptophan
-
 Daucus carota
4.1.3.27 DL-5-hydroxytryptophan
-
 Daucus carota
4.1.3.27 DL-5-Methyltryptophan 0.0054 mM, 50% inhibition Daucus carota
4.1.3.27 DL-6-Fluorotryptophan
-
 Daucus carota
4.1.3.27 Hg2+
-
 Daucus carota
4.1.3.27 L-Trp 0.0033 mM, 50% inhibition Daucus carota
4.1.3.27 Pb2+
-
 Daucus carota
4.1.3.27 PCMB
-
 Daucus carota
4.1.3.27 Zn2+
-
 Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.026
-
 chorismate wild-type enzyme Daucus carota
4.1.3.27 0.046
-
 chorismate mutant enzyme Daucus carota
4.1.3.27 0.237
-
 L-Gln mutant enzyme Daucus carota
4.1.3.27 0.249
-
 L-Gln wild-type enzyme Daucus carota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Co2+ can partially replace Mg2+ in activation Daucus carota
4.1.3.27 Mg2+ required Daucus carota
4.1.3.27 Mg2+ Km for the mutant enzyme: 0.036 mM, Km for the wild-type enzyme: 0.101 mM Daucus carota
4.1.3.27 Mn2+ can partially replace Mg2+ in activation Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Daucus carota
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.27 culture condition:DL-5-methyltryptophan-grown cell normal cells and cells resistant to growth inhibition by DL-5-methyltryptophan Daucus carota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Daucus carota anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+ 10% of the activity with Gln Daucus carota ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.27 0.0033
-
 L-Trp
-
 Daucus carota
4.1.3.27 0.0054
-
 DL-5-Methyltryptophan
-
 Daucus carota