BRENDA - Enzyme Database

Purification and properties of malyl-coenzyme A lyase from Pseudomonas AM1

Hacking, A.J.; Quayle, J.R.; Biochem. J. 139, 399-405 (1974)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.24
(2R)-4-malyl-CoA
-
Methylorubrum extorquens
4.1.3.24
iodoacetate
-
Methylorubrum extorquens
4.1.3.24
NEM
-
Methylorubrum extorquens
4.1.3.24
PCMB
-
Methylorubrum extorquens
4.1.3.24
Tris
-
Methylorubrum extorquens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.24
0.015
-
acetyl-CoA
-
Methylorubrum extorquens
4.1.3.24
0.066
-
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
Methylorubrum extorquens
4.1.3.24
1.7
-
glyoxylate
-
Methylorubrum extorquens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.24
Co2+
divalent cation required, Mg2+ or Co2+ is most effective
Methylorubrum extorquens
4.1.3.24
Mg2+
divalent cation required, Mg2+ or Co2+ is most effective; Km: 1.2 mM
Methylorubrum extorquens
4.1.3.24
Mn2+
divalent cation required, 88% of the activation with Mg2+ and Co2+
Methylorubrum extorquens
4.1.3.24
Zn2+
divalent cation required, 41% of the activation with Mg2+ and Co2+
Methylorubrum extorquens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.24
190000
-
meniscus depletion method
Methylorubrum extorquens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.24
Methylorubrum extorquens
-
-
-
4.1.3.24
Pseudomonas sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.24
-
Methylorubrum extorquens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.24
additional information
-
-
Methylorubrum extorquens
4.1.3.24
additional information
-
-
Pseudomonas sp.
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.24
-15C, stable for several months
Methylorubrum extorquens
4.1.3.24
0-4C, 2-3 weeks, stable
Methylorubrum extorquens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Pseudomonas sp.
acetyl-CoA + glyoxylate
-
33262
Pseudomonas sp.
r
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Methylorubrum extorquens
acetyl-CoA + glyoxylate
-
33262
Methylorubrum extorquens
r
4.1.3.24
acetyl-CoA + glyoxylate
-
33262
Pseudomonas sp.
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Pseudomonas sp.
r
4.1.3.24
acetyl-CoA + glyoxylate
-
33262
Methylorubrum extorquens
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Methylorubrum extorquens
r
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.3.24
55
-
irreversible loss of activity
Methylorubrum extorquens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.24
7.8
-
-
Methylorubrum extorquens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.24
6.5
10
pH 6.5: about 55% of maximal activity, pH 10.0: about 45% of maximal activity
Methylorubrum extorquens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.24
(2R)-4-malyl-CoA
-
Methylorubrum extorquens
4.1.3.24
iodoacetate
-
Methylorubrum extorquens
4.1.3.24
NEM
-
Methylorubrum extorquens
4.1.3.24
PCMB
-
Methylorubrum extorquens
4.1.3.24
Tris
-
Methylorubrum extorquens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.24
0.015
-
acetyl-CoA
-
Methylorubrum extorquens
4.1.3.24
0.066
-
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
Methylorubrum extorquens
4.1.3.24
1.7
-
glyoxylate
-
Methylorubrum extorquens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.24
Co2+
divalent cation required, Mg2+ or Co2+ is most effective
Methylorubrum extorquens
4.1.3.24
Mg2+
divalent cation required, Mg2+ or Co2+ is most effective; Km: 1.2 mM
Methylorubrum extorquens
4.1.3.24
Mn2+
divalent cation required, 88% of the activation with Mg2+ and Co2+
Methylorubrum extorquens
4.1.3.24
Zn2+
divalent cation required, 41% of the activation with Mg2+ and Co2+
Methylorubrum extorquens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.24
190000
-
meniscus depletion method
Methylorubrum extorquens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.24
-
Methylorubrum extorquens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.24
additional information
-
-
Methylorubrum extorquens
4.1.3.24
additional information
-
-
Pseudomonas sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.24
-15C, stable for several months
Methylorubrum extorquens
4.1.3.24
0-4C, 2-3 weeks, stable
Methylorubrum extorquens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Pseudomonas sp.
acetyl-CoA + glyoxylate
-
33262
Pseudomonas sp.
r
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Methylorubrum extorquens
acetyl-CoA + glyoxylate
-
33262
Methylorubrum extorquens
r
4.1.3.24
acetyl-CoA + glyoxylate
-
33262
Pseudomonas sp.
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Pseudomonas sp.
r
4.1.3.24
acetyl-CoA + glyoxylate
-
33262
Methylorubrum extorquens
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
33262
Methylorubrum extorquens
r
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.3.24
55
-
irreversible loss of activity
Methylorubrum extorquens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.24
7.8
-
-
Methylorubrum extorquens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.24
6.5
10
pH 6.5: about 55% of maximal activity, pH 10.0: about 45% of maximal activity
Methylorubrum extorquens