Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nakamura, K.; Amano, Y.; Nakadate, M.; Kagami, M.
    Purification and properties of isocitrate lyase from Candida brassicae E-17 (1989), J. Ferment. Bioeng., 67, 153-157.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.1 2-mercaptoethanol increase of activity Candida brassicae
4.1.3.1 cysteine increase of activity Candida brassicae
4.1.3.1 dithioerythritol increase of activity Candida brassicae
4.1.3.1 dithiothreitol increase of activity Candida brassicae
4.1.3.1 glutathione increase of activity Candida brassicae
4.1.3.1 thiol compounds increase of activity Candida brassicae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 2-mercaptoethanol increase of activity Candida brassicae
4.1.3.1 glutathione increase of activity Candida brassicae
4.1.3.1 glycolate
-
 Candida brassicae
4.1.3.1 malonate
-
 Candida brassicae
4.1.3.1 Mn2+ inhibition in presence of Mg2+, activation in absence of Mg2+ Candida brassicae
4.1.3.1 succinate
-
 Candida brassicae
4.1.3.1 Zn2+
-
 Candida brassicae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.62
-
 DL-isocitrate imidazole-HCl buffer pH 6.85 Candida brassicae
4.1.3.1 1.532
-
 DL-isocitrate sodium phosphate buffer, pH 6.85 Candida brassicae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Ba2+ Ba2+ less effective activator than Mg2+ Candida brassicae
4.1.3.1 Mg2+ maximal activity at 8 mM Candida brassicae
4.1.3.1 Mn2+ Mn2+ less effective activator than Mg2+ Candida brassicae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 290000
-
 gel filtration Candida brassicae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Candida brassicae glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Candida brassicae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Candida brassicae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 8.01
-
-
 Candida brassicae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Candida brassicae succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Candida brassicae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4*71000, SDS-PAGE Candida brassicae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.1 37
-
-
 Candida brassicae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 6.8
-
-
 Candida brassicae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.3.1 6 7 stable Candida brassicae