BRENDA - Enzyme Database

Purification and properties of isocitrate lyase from Aspergillus nidulans, a model enzyme to study catabolite inactivation in filamentous fungi

DeLucas, J.R.Amor.C.; Diaz, M.; Turner, G.; Laborda, F.; Mycol. Res. 101, 410-414 (1997)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
dithiothreitol
increase of activity; optimal concentration 1 mM
Aspergillus nidulans
4.1.3.1
thiol compounds
increase of activity
Aspergillus nidulans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
fructose-1,6-bisphosphate
-
Aspergillus nidulans
4.1.3.1
malonate
-
Aspergillus nidulans
4.1.3.1
methylenesuccinate
-
Aspergillus nidulans
4.1.3.1
oxalate
-
Aspergillus nidulans
4.1.3.1
succinate
-
Aspergillus nidulans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.05
-
threo-Ds-isocitrate
-
Aspergillus nidulans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 18% as effective as Mg2+; no activity in absence of exogenous cation
Aspergillus nidulans
4.1.3.1
Mg2+
required for full activity, optimal concentration 5 mM
Aspergillus nidulans
4.1.3.1
Mn2+
Mn2+ 31% as effective as Mg2+; no activity in absence of exogenous cation
Aspergillus nidulans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
59000
-
4 * 59000, SDS-PAGE
Aspergillus nidulans
4.1.3.1
240000
-
gel filtration
Aspergillus nidulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Aspergillus nidulans
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Aspergillus nidulans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Aspergillus nidulans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
11
-
-
Aspergillus nidulans
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, several weeks, stable
Aspergillus nidulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33141
Aspergillus nidulans
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33141
Aspergillus nidulans
succinate + glyoxylate
-
33141
Aspergillus nidulans
r
4.1.3.1
isocitrate
glyoxylate cycle
33141
Aspergillus nidulans
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 59000, SDS-PAGE
Aspergillus nidulans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
40
50
stable for 1 h
Aspergillus nidulans
4.1.3.1
50
-
completely inactive at temperatures above 50°C
Aspergillus nidulans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
dithiothreitol
increase of activity; optimal concentration 1 mM
Aspergillus nidulans
4.1.3.1
thiol compounds
increase of activity
Aspergillus nidulans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
fructose-1,6-bisphosphate
-
Aspergillus nidulans
4.1.3.1
malonate
-
Aspergillus nidulans
4.1.3.1
methylenesuccinate
-
Aspergillus nidulans
4.1.3.1
oxalate
-
Aspergillus nidulans
4.1.3.1
succinate
-
Aspergillus nidulans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.05
-
threo-Ds-isocitrate
-
Aspergillus nidulans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 18% as effective as Mg2+; no activity in absence of exogenous cation
Aspergillus nidulans
4.1.3.1
Mg2+
required for full activity, optimal concentration 5 mM
Aspergillus nidulans
4.1.3.1
Mn2+
Mn2+ 31% as effective as Mg2+; no activity in absence of exogenous cation
Aspergillus nidulans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
59000
-
4 * 59000, SDS-PAGE
Aspergillus nidulans
4.1.3.1
240000
-
gel filtration
Aspergillus nidulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Aspergillus nidulans
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Aspergillus nidulans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
11
-
-
Aspergillus nidulans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, several weeks, stable
Aspergillus nidulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33141
Aspergillus nidulans
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33141
Aspergillus nidulans
succinate + glyoxylate
-
33141
Aspergillus nidulans
r
4.1.3.1
isocitrate
glyoxylate cycle
33141
Aspergillus nidulans
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 59000, SDS-PAGE
Aspergillus nidulans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
40
50
stable for 1 h
Aspergillus nidulans
4.1.3.1
50
-
completely inactive at temperatures above 50°C
Aspergillus nidulans