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Literature summary extracted from

  • Hoyt, J.C.; Johnson, K.E.; Reeves, H.C.
    Purification and characterization of Acinetobacter calcoaceticus isocitrate lyase (1991), J. Bacteriol., 173, 6844-6848.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 Ba2+
-
 Acinetobacter calcoaceticus
4.1.3.1 Ca2+
-
 Acinetobacter calcoaceticus
4.1.3.1 glycolate
-
 Acinetobacter calcoaceticus
4.1.3.1 Hydroxymalonate
-
 Acinetobacter calcoaceticus
4.1.3.1 malonate
-
 Acinetobacter calcoaceticus
4.1.3.1 methylenesuccinate
-
 Acinetobacter calcoaceticus
4.1.3.1 oxalate
-
 Acinetobacter calcoaceticus
4.1.3.1 Sr2+
-
 Acinetobacter calcoaceticus
4.1.3.1 Tartrate
-
 Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.04
-
 threo-Ds-isocitrate
-
 Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Co2+ no activity in absence of exogenous cation Acinetobacter calcoaceticus
4.1.3.1 Co2+ Co2+ 12% as effective as Mg2+ Acinetobacter calcoaceticus
4.1.3.1 Fe2+ no activity in absence of exogenous cation, Fe2+ 7% as effective as Mg2+ Acinetobacter calcoaceticus
4.1.3.1 Mg2+ increase of activity Acinetobacter calcoaceticus
4.1.3.1 Mn2+ no activity in absence of exogenous cation Acinetobacter calcoaceticus
4.1.3.1 Mn2+ Mn2+ 14% as effective as Mg2+ Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 64000
-
 4 * 64000, SDS-PAGE Acinetobacter calcoaceticus
4.1.3.1 250000
-
 gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Acinetobacter calcoaceticus glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 2.9
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R,3S)-isocitrate
-
 Acinetobacter calcoaceticus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Acinetobacter calcoaceticus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Acinetobacter calcoaceticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4 * 64000, SDS-PAGE Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7.5
-
-
 Acinetobacter calcoaceticus