BRENDA - Enzyme Database

Isolation and characterization of isocitrate lyase from a thermophilic Bacillus sp.

Chell, R.M.; Sundaram, T.K.; Wilkinson, A.E.; Biochem. J. 173, 165-177 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Maleate
condensation reaction
Bacillus sp. (in: Bacteria)
4.1.3.1
malonate
condensation reaction
Bacillus sp. (in: Bacteria)
4.1.3.1
methylenesuccinate
condensation reaction
Bacillus sp. (in: Bacteria)
4.1.3.1
p-chloromercuribenzoate
100% inhibition at 0.02 mM, reversible by 2-mercaptoethanol
Bacillus sp. (in: Bacteria)
4.1.3.1
phosphoenolpyruvate
-
Bacillus sp. (in: Bacteria)
4.1.3.1
succinate
-
Bacillus sp. (in: Bacteria)
4.1.3.1
Urea
partial inactivation
Bacillus sp. (in: Bacteria)
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.02
-
threo-Ds-isocitrate
25 mM imidazole buffer, pH 6.8
Bacillus sp. (in: Bacteria)
4.1.3.1
0.2
-
threo-Ds-isocitrate
0.4 M KCl, 25 mM imidazole buffer, pH 6.8
Bacillus sp. (in: Bacteria)
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
absolute requirement
Bacillus sp. (in: Bacteria)
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
48000
-
4 * 48000, SDS-PAGE
Bacillus sp. (in: Bacteria)
4.1.3.1
180000
-
sedimentation equilibrium centrifugation, gel filtration
Bacillus sp. (in: Bacteria)
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Bacillus sp. (in: Bacteria)
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Bacillus sp. (in: Bacteria)
-
thermophilic
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
thermophilic
Bacillus sp. (in: Bacteria)
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
2.9
-
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33135
Bacillus sp. (in: Bacteria)
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33135
Bacillus sp. (in: Bacteria)
succinate + glyoxylate
-
33135
Bacillus sp. (in: Bacteria)
r
4.1.3.1
isocitrate
glyoxylate cycle
33135
Bacillus sp. (in: Bacteria)
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 48000, SDS-PAGE
Bacillus sp. (in: Bacteria)
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.1.3.1
additional information
-
KCl, MgCl2, CaCl2 stabilize
Bacillus sp. (in: Bacteria)
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
24
-
threo-Ds-isocitrate
-
Bacillus sp. (in: Bacteria)
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Maleate
condensation reaction
Bacillus sp. (in: Bacteria)
4.1.3.1
malonate
condensation reaction
Bacillus sp. (in: Bacteria)
4.1.3.1
methylenesuccinate
condensation reaction
Bacillus sp. (in: Bacteria)
4.1.3.1
p-chloromercuribenzoate
100% inhibition at 0.02 mM, reversible by 2-mercaptoethanol
Bacillus sp. (in: Bacteria)
4.1.3.1
phosphoenolpyruvate
-
Bacillus sp. (in: Bacteria)
4.1.3.1
succinate
-
Bacillus sp. (in: Bacteria)
4.1.3.1
Urea
partial inactivation
Bacillus sp. (in: Bacteria)
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.02
-
threo-Ds-isocitrate
25 mM imidazole buffer, pH 6.8
Bacillus sp. (in: Bacteria)
4.1.3.1
0.2
-
threo-Ds-isocitrate
0.4 M KCl, 25 mM imidazole buffer, pH 6.8
Bacillus sp. (in: Bacteria)
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
absolute requirement
Bacillus sp. (in: Bacteria)
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
48000
-
4 * 48000, SDS-PAGE
Bacillus sp. (in: Bacteria)
4.1.3.1
180000
-
sedimentation equilibrium centrifugation, gel filtration
Bacillus sp. (in: Bacteria)
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Bacillus sp. (in: Bacteria)
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
thermophilic
Bacillus sp. (in: Bacteria)
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
2.9
-
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33135
Bacillus sp. (in: Bacteria)
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33135
Bacillus sp. (in: Bacteria)
succinate + glyoxylate
-
33135
Bacillus sp. (in: Bacteria)
r
4.1.3.1
isocitrate
glyoxylate cycle
33135
Bacillus sp. (in: Bacteria)
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 48000, SDS-PAGE
Bacillus sp. (in: Bacteria)
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.1.3.1
additional information
-
KCl, MgCl2, CaCl2 stabilize
Bacillus sp. (in: Bacteria)
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
24
-
threo-Ds-isocitrate
-
Bacillus sp. (in: Bacteria)