BRENDA - Enzyme Database

Studies on isocitrate lyase isolated from Lupinus cotyledons

Vanni, P.; Vicenzini, M.T.; Nerozzi, F.M.; Sinha, S.P.; Can. J. Biochem. 57, 1131-1137 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
malonate
-
Lupinus sp.
4.1.3.1
N-ethylmaleimide
-
Lupinus sp.
4.1.3.1
oxalate
-
Lupinus sp.
4.1.3.1
p-chloromercuribenzoate
-
Lupinus sp.
4.1.3.1
phosphoenolpyruvate
-
Lupinus sp.
4.1.3.1
succinate
-
Lupinus sp.
4.1.3.1
Tartrate
-
Lupinus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.1
0.2
isocitrate
Tris, triethanolamine or imidazole buffer
Lupinus sp.
4.1.3.1
1
-
isocitrate
phosphate buffer, pH 6.8 or 7.5
Lupinus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
-
Lupinus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
145000
170000
gel filtration
Lupinus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Lupinus sp.
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Lupinus sp.
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.1.3.1
reducing agent necessary for maintenance of enzyme activity
Lupinus sp.
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Lupinus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Lupinus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
3.68
-
-
Lupinus sp.
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-30°C, 0.3 mM dithiothreitol, 12 days, 50% loss of activity
Lupinus sp.
4.1.3.1
-30°C, 12 days, almost all activity lost
Lupinus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33131
Lupinus sp.
succinate + glyoxylate
-
33131
Lupinus sp.
r
4.1.3.1
isocitrate
glyoxylate cycle
33131
Lupinus sp.
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7
-
triethanolamine buffer
Lupinus sp.
4.1.3.1
7.5
-
phosphate, Tris or imidazole buffer
Lupinus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
malonate
-
Lupinus sp.
4.1.3.1
N-ethylmaleimide
-
Lupinus sp.
4.1.3.1
oxalate
-
Lupinus sp.
4.1.3.1
p-chloromercuribenzoate
-
Lupinus sp.
4.1.3.1
phosphoenolpyruvate
-
Lupinus sp.
4.1.3.1
succinate
-
Lupinus sp.
4.1.3.1
Tartrate
-
Lupinus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.1
0.2
isocitrate
Tris, triethanolamine or imidazole buffer
Lupinus sp.
4.1.3.1
1
-
isocitrate
phosphate buffer, pH 6.8 or 7.5
Lupinus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
-
Lupinus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
145000
170000
gel filtration
Lupinus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Lupinus sp.
glyoxylate cycle
?
-
-
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.1.3.1
reducing agent necessary for maintenance of enzyme activity
Lupinus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Lupinus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Lupinus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
3.68
-
-
Lupinus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-30°C, 0.3 mM dithiothreitol, 12 days, 50% loss of activity
Lupinus sp.
4.1.3.1
-30°C, 12 days, almost all activity lost
Lupinus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33131
Lupinus sp.
succinate + glyoxylate
-
33131
Lupinus sp.
r
4.1.3.1
isocitrate
glyoxylate cycle
33131
Lupinus sp.
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7
-
triethanolamine buffer
Lupinus sp.
4.1.3.1
7.5
-
phosphate, Tris or imidazole buffer
Lupinus sp.