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Literature summary extracted from

  • Vicenzini, M.T.; Nerozzi, F.; Vincieri, F.F.; Vanni, P.
    Isolation and properties of isocitrate lyase from Lupinus seed (1980), Phytochemistry, 19, 769-774.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.1 dithiothreitol increase of activity Lupinus sp.
4.1.3.1 Na2S2O4 increase of activity Lupinus sp.
4.1.3.1 Reducing agents increase of activity Lupinus sp.
4.1.3.1 thioglycolate increase of activity Lupinus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 glyoxylate
-
 Lupinus sp.
4.1.3.1 malonate
-
 Lupinus sp.
4.1.3.1 N-ethylmaleimide
-
 Lupinus sp.
4.1.3.1 oxalate
-
 Lupinus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.05
-
 glyoxylate
-
 Lupinus sp.
4.1.3.1 0.1 0.2 isocitrate Tris, triethanolamine or imidazole buffer Lupinus sp.
4.1.3.1 0.2
-
 succinate
-
 Lupinus sp.
4.1.3.1 1
-
 isocitrate phosphate buffer, pH 6.8 or 7.5 Lupinus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ increase of activity Lupinus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 145000 170000 gel filtration Lupinus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Lupinus sp. glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Lupinus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Lupinus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 seed
-
 Lupinus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 1.83
-
-
 Lupinus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Lupinus sp. succinate + glyoxylate
-
 r
4.1.3.1 isocitrate glyoxylate cycle Lupinus sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7
-
 triethanolamine buffer Lupinus sp.
4.1.3.1 7.5
-
 phosphate, Tris or imidazole buffer Lupinus sp.