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Literature summary extracted from

  • Mackintosh, C.; Nimmo, H.G.
    Purification and regulatory properties of isocitrate lyase from Escherichia coli ML308 (1988), Biochem. J., 250, 25-31.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 2-oxoglutarate
-
 Escherichia coli
4.1.3.1 3-phosphoglycerate
-
 Escherichia coli
4.1.3.1 Cl-
-
 Escherichia coli
4.1.3.1 glycolate
-
 Escherichia coli
4.1.3.1 phosphate
-
 Escherichia coli
4.1.3.1 phosphoenolpyruvate
-
 Escherichia coli
4.1.3.1 SO42-
-
 Escherichia coli
4.1.3.1 succinate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.062
-
 Ds-isocitrate pH 7.3 Escherichia coli
4.1.3.1 0.13
-
 glyoxylate
-
 Escherichia coli
4.1.3.1 0.59
-
 succinate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 44700
-
 4 * 44700, SDS-PAGE Escherichia coli
4.1.3.1 177000
-
 gel filtration Escherichia coli
4.1.3.1 180000
-
 sedimentation equilibrium centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 37.5
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 -20°C, at least 6 months, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Escherichia coli succinate + glyoxylate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4 * 44700, SDS-PAGE Escherichia coli