BRENDA - Enzyme Database

Escherichia coli isocitrate lyase: properties and comparisons

Hoyt, J.C.; Robertson, E.F.; Berlyn, K.A.; Reeves, H.C.; Biochim. Biophys. Acta 966, 30-35 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
3-nitropropionate
50% inhibition at 0.04 mM
Escherichia coli
4.1.3.1
Ca2+
-
Escherichia coli
4.1.3.1
Dithionitrobenzoate
-
Escherichia coli
4.1.3.1
glycolate
50% inhibition at 5 mM
Escherichia coli
4.1.3.1
HgCl2
100% inhibition at 0.01 mM
Escherichia coli
4.1.3.1
Hydroxymalonate
50% inhibition at 0.5 mM
Escherichia coli
4.1.3.1
KCl
-
Escherichia coli
4.1.3.1
malonate
-
Escherichia coli
4.1.3.1
methylmalonate
50% inhibition at 15 mM
Escherichia coli
4.1.3.1
NaCl
-
Escherichia coli
4.1.3.1
oxalate
-
Escherichia coli
4.1.3.1
succinate
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 17% as effective as Mg2+; no activity in absence of exogenous cation
Escherichia coli
4.1.3.1
Mg2+
no activity in absence of exogenous cation, Mg2+ most effective
Escherichia coli
4.1.3.1
Mn2+
Mn2+ 54% as effective as Mg2+; no activity in absence of exogenous cation
Escherichia coli
4.1.3.1
Ni2+
no activity in absence of exogenous cation, Ni2+ 7% as effective as Mg2+
Escherichia coli
4.1.3.1
Sr2+
no activity in absence of exogenous cation, Sr2+ 3% as effective as Mg2+
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Escherichia coli
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33117
Escherichia coli
succinate + glyoxylate
-
33117
Escherichia coli
r
4.1.3.1
isocitrate
glyoxylate cycle
33117
Escherichia coli
?
-
-
-
-
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.04
-
3-nitropropionate
-
Escherichia coli
4.1.3.1
0.5
-
Hydroxymalonate
-
Escherichia coli
4.1.3.1
15
-
methylmalonate
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
3-nitropropionate
50% inhibition at 0.04 mM
Escherichia coli
4.1.3.1
Ca2+
-
Escherichia coli
4.1.3.1
Dithionitrobenzoate
-
Escherichia coli
4.1.3.1
glycolate
50% inhibition at 5 mM
Escherichia coli
4.1.3.1
HgCl2
100% inhibition at 0.01 mM
Escherichia coli
4.1.3.1
Hydroxymalonate
50% inhibition at 0.5 mM
Escherichia coli
4.1.3.1
KCl
-
Escherichia coli
4.1.3.1
malonate
-
Escherichia coli
4.1.3.1
methylmalonate
50% inhibition at 15 mM
Escherichia coli
4.1.3.1
NaCl
-
Escherichia coli
4.1.3.1
oxalate
-
Escherichia coli
4.1.3.1
succinate
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.04
-
3-nitropropionate
-
Escherichia coli
4.1.3.1
0.5
-
Hydroxymalonate
-
Escherichia coli
4.1.3.1
15
-
methylmalonate
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 17% as effective as Mg2+; no activity in absence of exogenous cation
Escherichia coli
4.1.3.1
Mg2+
no activity in absence of exogenous cation, Mg2+ most effective
Escherichia coli
4.1.3.1
Mn2+
Mn2+ 54% as effective as Mg2+; no activity in absence of exogenous cation
Escherichia coli
4.1.3.1
Ni2+
no activity in absence of exogenous cation, Ni2+ 7% as effective as Mg2+
Escherichia coli
4.1.3.1
Sr2+
no activity in absence of exogenous cation, Sr2+ 3% as effective as Mg2+
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Escherichia coli
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33117
Escherichia coli
succinate + glyoxylate
-
33117
Escherichia coli
r
4.1.3.1
isocitrate
glyoxylate cycle
33117
Escherichia coli
?
-
-
-
-