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Literature summary extracted from

  • Lochmueller, H.; Wood, H.G.; Davis, J.J.
    Phosphoenolpyruvate carboxytransphosphorylase. II. Crystallization and properties (1966), J. Biol. Chem., 241, 5678-5691.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.38
-
 Propionibacterium freudenreichii subsp. shermanii

General Stability

EC Number General Stability Organism
4.1.1.38 0.05 M phosphate buffer, 0.01 M or 0.001 M thiol, 0.01 mg protein per ml, stable Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 30 h dialysis against phosphate buffer containing 1 mM mercaptoethanol, with a change of buffer at 15 h, stable Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.38 Ca2+
-
 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 diphosphate at 1 mM Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 DL-malate
-
 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 EDTA
-
 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 glycylglycine higher inhibition at pH 7.8 than at pH 6.5 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 KCl at pH 6.5 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 MgCl2 at 7 mM Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 NaCl at pH 6.5 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 oxaloacetate at 7 mM Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 PCMB
-
 Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 SO42- competitive against phosphate Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 Tris-HCl higher inhibition at pH 7.8 than at pH 6.5 Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.38 Co2+ can partially replace Mn2+ or Mg2+ in activation Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 Mg2+ Mn2+ or Mg2+ required Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 Mn2+ Mn2+ or Mg2+ required Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.38 430000
-
 low speed sedimentation without reaching equilibrium Propionibacterium freudenreichii subsp. shermanii

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.38 Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.38
-
 Propionibacterium freudenreichii subsp. shermanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.38 additional information
-
-
 Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.38 phosphate + phosphoenolpyruvate + CO2
-
 Propionibacterium freudenreichii subsp. shermanii diphosphate + oxaloacetate
-
 r
4.1.1.38 phosphoenolpyruvate + phosphate reaction is catalyzed in absence of CO2 Propionibacterium freudenreichii subsp. shermanii pyruvate + diphosphate
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.38 6.1 8 formation of oxaloacetate Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 7 7.5 formation of phosphoenolpyruvate from oxaloacetate and diphosphate Propionibacterium freudenreichii subsp. shermanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.38 5.5 8.5 pH 5.5: about 35% of maximal activity, pH 8.5: about 55% of maximal activity, formation of oxalacetate Propionibacterium freudenreichii subsp. shermanii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.38 4
-
 inactivation, even at 0°C Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 4.5
-
 25°C, 5 h, little loss of activity Propionibacterium freudenreichii subsp. shermanii
4.1.1.38 8.2
-
 25°C, 0.2 M NaHCO3, 60% loss of activity after 30 min, rapid loss of activity above pH 8.2 Propionibacterium freudenreichii subsp. shermanii