Literature summary extracted from
Yang, G.; Liu, R.Q.; Taylor, K.L.; Xiang, H.; Price, J.; Dunaway-Mariano, D.
Identification of active site residues essential to 4-chlorobenzoyl-coenzyme A dehalogenase catalysis by chemical modification and site directed mutagenesis (1996), Biochemistry, 35, 10879-10885.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.8.1.7 |
H81Q |
mutant enzymes H81Q, W137F and H90Q show significant loss in catalytic activity |
Pseudomonas sp. |
3.8.1.7 |
H94Q |
The mutant enzymes H94Q, H208Q, and W179F have a catalytic activity comparable to the wild-type enzyme |
Pseudomonas sp. |
3.8.1.7 |
W137F |
mutant enzymes H81Q, W137F and H90Q show significant loss in catalytic activity |
Pseudomonas sp. |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.8.1.7 |
diethyl dicarbonate |
activity is fully regained by subsequent treatment with hydroxylamine |
Pseudomonas sp. |
|
3.8.1.7 |
N-bromosuccinimide |
- |
Pseudomonas sp. |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.8.1.7 |
Pseudomonas sp. |
- |
recombinant enzyme expressed in Escherichia coli |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.8.1.7 |
4-chlorobenzoyl-CoA + H2O = 4-hydroxybenzoyl CoA + chloride |
it is proposed that Asp125 functions as the active site nucleophile, that Trp137 serves as a hydrogen bond donor to the Asp145 carbonyl group , and that His90 serves to deprotonate the bound H2O molecule |
Pseudomonas sp. |
|