EC Number | General Stability | Organism |
---|---|---|
3.8.1.7 | pure enzyme is completely inactive after a single freeze and thawing cycle | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.8.1.7 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Pseudomonas sp. | |
3.8.1.7 | Ag+ | 5 mM | Pseudomonas sp. | |
3.8.1.7 | Co2+ | 5 mM | Pseudomonas sp. | |
3.8.1.7 | Cu2+ | 5 mM | Pseudomonas sp. | |
3.8.1.7 | Fe2+ | 5 mM | Pseudomonas sp. | |
3.8.1.7 | Mn2+ | 5 mM | Pseudomonas sp. | |
3.8.1.7 | Ni2+ | 5 mM | Pseudomonas sp. | |
3.8.1.7 | SDS | 1 mM, 80% inhibition | Pseudomonas sp. | |
3.8.1.7 | Zn2+ | 5 mM | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.7 | 0.0024 | 0.0027 | 4-chlorobenzoyl-CoA | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 12000 | - |
gel filtration | Pseudomonas sp. |
3.8.1.7 | 31000 | - |
4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
3.8.1.7 | 31000 | - |
8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
3.8.1.7 | 31000 | - |
x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.7 | Pseudomonas sp. | - |
- |
- |
3.8.1.7 | Pseudomonas sp. CBS-3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.8.1.7 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 2.25 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.8.1.7 | -20°C, impure preparations, stable for several months | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.7 | 4-bromobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + bromide | - |
? | |
3.8.1.7 | 4-bromobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + bromide | - |
? | |
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + chloride | - |
? | |
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + chloride | - |
? | |
3.8.1.7 | 4-fluorobenzoyl-CoA + H2O | no activity | Pseudomonas sp. | 4-hydroxybenzoyl-CoA + fluoride | - |
? | |
3.8.1.7 | 4-fluorobenzoyl-CoA + H2O | no activity | Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + fluoride | - |
? | |
3.8.1.7 | 4-iodobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + iodide | - |
? | |
3.8.1.7 | 4-iodobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + iodide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.8.1.7 | multimer | x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
3.8.1.7 | octamer | 8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
3.8.1.7 | tetramer | 4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 60 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 30 | 65 | about 60% of maximal activity at 30°C and at 65°C | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 50 | - |
15 min, stable | Pseudomonas sp. |
3.8.1.7 | 60 | - |
15 min, 30% loss of activity | Pseudomonas sp. |
3.8.1.7 | 65 | - |
15 min, complete loss of activity | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.7 | 5 | - |
4-chlorobenzoyl-CoA | - |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 10 | - |
- |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 9 | 11.5 | about 50% of maximal activity at pH 9.0 and at pH 11.5 | Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.8.1.7 | 4.5 | - |
10 min, complete loss of activity | Pseudomonas sp. |
3.8.1.7 | 6 | - |
60 min, 10% loss of activity | Pseudomonas sp. |
3.8.1.7 | 6.5 | 11 | 60 min, stable | Pseudomonas sp. |
3.8.1.7 | 11.5 | - |
60 min, 10% loss of activity | Pseudomonas sp. |
3.8.1.7 | 12 | - |
10 min, complete loss of activity | Pseudomonas sp. |