BRENDA - Enzyme Database

Purification and characterization of 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain 4-CB1

Crooks, G.P.; Copley, S.D.; Biochemistry 33, 11645-11649 (1994)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.014
-
4-iodobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.034
-
4-chlorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.036
-
4-bromobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.04
-
4-fluorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.075
-
4-fluorobenzoyl-CoA
-
Arthrobacter sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.8.1.7
Arthrobacter sp.
-
-
-
3.8.1.7
Arthrobacter sp. 4-CB1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.8.1.7
-
Arthrobacter sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.8.1.7
additional information
-
-
Arthrobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.8.1.7
4-bromobenzoyl-CoA + H2O
-
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + bromide
-
-
-
-
3.8.1.7
4-bromobenzoyl-CoA + H2O
-
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + bromide
-
-
-
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + chloride
-
33077
Arthrobacter sp.
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + chloride
-
33077
Arthrobacter sp. 4-CB1
-
3.8.1.7
4-fluorobenzoyl-CoA + H2O
weak activity
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + fluoride
-
-
-
-
3.8.1.7
4-fluorobenzoyl-CoA + H2O
weak activity
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + fluoride
-
-
-
-
3.8.1.7
4-iodobenzoyl-CoA + H2O
-
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + iodide
-
-
-
-
3.8.1.7
4-iodobenzoyl-CoA + H2O
-
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + iodide
-
-
-
-
3.8.1.7
additional information
no activity with 3-chlorobenzoyl-CoA and 2-chlorobenzoyl-CoA
33077
Arthrobacter sp.
?
-
-
-
-
3.8.1.7
additional information
no activity with 3-chlorobenzoyl-CoA and 2-chlorobenzoyl-CoA
33077
Arthrobacter sp. 4-CB1
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.003
-
4-fluorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
1.3
-
4-chlorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
2
-
4-iodobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
2.4
-
4-bromobenzoyl-CoA
-
Arthrobacter sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8.1.7
8
-
-
Arthrobacter sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.8.1.7
6.5
10
4░C, 4 h, stable
Arthrobacter sp.
3.8.1.7
6.5
-
irreversible inactivation below
Arthrobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.014
-
4-iodobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.034
-
4-chlorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.036
-
4-bromobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.04
-
4-fluorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
0.075
-
4-fluorobenzoyl-CoA
-
Arthrobacter sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.8.1.7
-
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.8.1.7
additional information
-
-
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.8.1.7
4-bromobenzoyl-CoA + H2O
-
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + bromide
-
-
-
-
3.8.1.7
4-bromobenzoyl-CoA + H2O
-
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + bromide
-
-
-
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + chloride
-
33077
Arthrobacter sp.
-
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + chloride
-
33077
Arthrobacter sp. 4-CB1
-
3.8.1.7
4-fluorobenzoyl-CoA + H2O
weak activity
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + fluoride
-
-
-
-
3.8.1.7
4-fluorobenzoyl-CoA + H2O
weak activity
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + fluoride
-
-
-
-
3.8.1.7
4-iodobenzoyl-CoA + H2O
-
33077
Arthrobacter sp.
4-hydroxybenzoyl-CoA + iodide
-
-
-
-
3.8.1.7
4-iodobenzoyl-CoA + H2O
-
33077
Arthrobacter sp. 4-CB1
4-hydroxybenzoyl-CoA + iodide
-
-
-
-
3.8.1.7
additional information
no activity with 3-chlorobenzoyl-CoA and 2-chlorobenzoyl-CoA
33077
Arthrobacter sp.
?
-
-
-
-
3.8.1.7
additional information
no activity with 3-chlorobenzoyl-CoA and 2-chlorobenzoyl-CoA
33077
Arthrobacter sp. 4-CB1
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.003
-
4-fluorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
1.3
-
4-chlorobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
2
-
4-iodobenzoyl-CoA
-
Arthrobacter sp.
3.8.1.7
2.4
-
4-bromobenzoyl-CoA
-
Arthrobacter sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8.1.7
8
-
-
Arthrobacter sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.8.1.7
6.5
10
4░C, 4 h, stable
Arthrobacter sp.
3.8.1.7
6.5
-
irreversible inactivation below
Arthrobacter sp.