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Literature summary extracted from

  • Orsomando, G.; Polzonetti, V.; Natalini, P.
    NAD(P)+-glycohydrolase from human spleen: a multicatalytic enzyme (2000), Comp. Biochem. Physiol. B, 126, 89-98.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.6 50% inactivation at 2 M urea Homo sapiens
3.2.2.6 SDS, even at micromolar concentration, leads to complete inactivation of the enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.6 Ag+ 50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity Homo sapiens
3.2.2.6 Cr3+ inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
3.2.2.6 Hg2+ complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
3.2.2.6 Pb2+ complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
3.2.2.6 SDS
-
 Homo sapiens
3.2.2.6 Sn2+ complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
3.2.2.6 Zn2+ inhibition of NADase and adenosine diphosphate cyclase Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.6 0.026
-
 NAD+
-
 Homo sapiens
3.2.2.6 0.026
-
 NAD+ pH 7.0, 28°C Homo sapiens
3.2.2.6 0.033
-
 epsilonNAD+
-
 Homo sapiens
3.2.2.6 0.033
-
 nicotinamide 1,N6-ethenoadenine dinucleotide pH 7.0, 28°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.6 membrane
-
 Homo sapiens 16020
-
3.2.2.6 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.6 Ag+ 50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase Homo sapiens
3.2.2.6 Ag+ at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity Homo sapiens
3.2.2.6 Cu2+ stimulation of cADPR activity Homo sapiens
3.2.2.6 additional information no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.6 additional information
-
 native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column Homo sapiens
3.2.2.6 45000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.6 additional information Homo sapiens enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.6 partial Homo sapiens
3.2.2.6 partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.6 NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ also catalyses transfer of ADP-ribose(P) residues Homo sapiens
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.2.6 80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.6 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.6 0.02
-
 partially purified enzyme, ADPR activity Homo sapiens
3.2.2.6 0.113
-
 partially purified enzyme, cADPR activity Homo sapiens
3.2.2.6 2.205
-
 partially purified enzyme, NADase activity Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.2.6 20°C, lyophilized enzyme, 90% remaining activity after 6 months Homo sapiens
3.2.2.6 4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable Homo sapiens
3.2.2.6 4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.6 3-acetylpyridine + adenosine diphosphoribose transglycosylation activity, very poor hydrolytic activity with this substrate Homo sapiens 3-acetylpyridine adenine dinucleotide + phosphate
-
 ?
3.2.2.6 3-acetylpyridine adenine dinucleotide + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 3-acetylpyridine hypoxanthine dinucleotide + H2O low activity Homo sapiens ?
-
 ?
3.2.2.6 3-pyridine adenine dinucleotide + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 3-pyridinealdehyde hypoxanthine dinucleotide + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 3-pyridinealdehyde hypoxanthine dinucleotide + H2O 60% activity compared to the activity with NAD+ Homo sapiens ?
-
 ?
3.2.2.6 cyclic ADP-ribose + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 cyclic ADP-ribose + H2O i.e. ADPR activity Homo sapiens adenosine diphosphoribose + ?
-
 ?
3.2.2.6 epsilon-NAD+ + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 epsilon-NADP+ + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 additional information enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose Homo sapiens ?
-
 ?
3.2.2.6 additional information enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose Homo sapiens ?
-
 ?
3.2.2.6 NAD+
-
 Homo sapiens cyclic ADP-ribose + nicotinamide
-
 ?
3.2.2.6 NAD+ + ? i.e. cADPR activity Homo sapiens cyclic ADP-ribose + ?
-
 ?
3.2.2.6 NAD+ + H2O
-
 Homo sapiens ADP-ribose + nicotinamide
-
 ?
3.2.2.6 NADP+ + H2O
-
 Homo sapiens nicotinamide + ADPribose-phosphate
-
 ?
3.2.2.6 NADP+ + H2O 80% activity compared to the activity with NAD+ Homo sapiens ADP-ribose-P + nicotinamide
-
 ?
3.2.2.6 NGD+ + H2O as effective as substrate as NAD+ Homo sapiens GDP-ribose + nicotinamide
-
 ?
3.2.2.6 nicotinamide 1,N6-ethenoadenine dinucleotide + H2O i.e. epsilonNAD+, used for a fluorometric assay Homo sapiens ?
-
 ?
3.2.2.6 nicotinamide guanine dinucleotide + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 nicotinamide-hypoxanthine dinucleotide + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.6 thio-NAD+ + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.6 oligomer x * 45000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.2.6 NAD(P)+-glycohydrolase
-
 Homo sapiens
3.2.2.6 NADase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.6 38
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.6 38 50 inactive above 50°C Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.6 38
-
 half-life: about 1 day Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.6 7
-
-
 Homo sapiens
3.2.2.6 7
-
 NADase activity Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.6 4.5 9.5
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.2.6 Homo sapiens isoelectric focusing
-
 4.9