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Literature summary extracted from
- Purification and properties of a beta-1,6-glucosidase from Flavobacterium (1975), Biochim. Biophys. Acta, 377, 410-420.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Ag+ | strong inhibition at 2 mM | Flavobacterium sp. |  |
| 3.2.1.21 | beta-D-glucose | - |
Flavobacterium sp. | |
| 3.2.1.21 | Cu2+ | strong inhibition at 2 mM | Flavobacterium sp. | |
| 3.2.1.21 | D-glucono-1,5-lactone | - |
Flavobacterium sp. | |
| 3.2.1.21 | Fe3+ | strong inhibition at 2 mM | Flavobacterium sp. | |
| 3.2.1.21 | Hg2+ | strong inhibition at 2 mM | Flavobacterium sp. | |
| 3.2.1.21 | N-ethylmaleimide | - |
Flavobacterium sp. | |
| 3.2.1.21 | p-chloromercuribenzoic acid | - |
Flavobacterium sp. | |
| 3.2.1.21 | Tris | inhibitory effect | Flavobacterium sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | additional information | - |
luteose | in mg/ml | Flavobacterium sp. | |
| 3.2.1.21 | 0.28 | - |
gentiobiose | - |
Flavobacterium sp. | |
| 3.2.1.21 | 0.3 | - |
Gentiotriose | - |
Flavobacterium sp. | |
| 3.2.1.21 | 0.42 | - |
Gentiotetraose | - |
Flavobacterium sp. | |
| 3.2.1.21 | 0.46 | - |
Gentiopentaose | - |
Flavobacterium sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | intracellular | - |
Flavobacterium sp. | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 49000 | - |
gel filtration, SDS-PAGE | Flavobacterium sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Flavobacterium sp. | very low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Flavobacterium sp. | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Flavobacterium sp. M64 | very low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Flavobacterium sp. M64 | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Flavobacterium sp. | gentiobiose | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Flavobacterium sp. | transglycosylation reaction | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Flavobacterium sp. M64 | gentiobiose | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Flavobacterium sp. M64 | transglycosylation reaction | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | gentiopentaose + 4 H2O | Flavobacterium sp. | specific for beta-1,6-linkages, activity decreases with number of glucose residues | 5 D-glucose | - |
? | |
| 3.2.1.21 | gentiotetraose + 3 H2O | Flavobacterium sp. | - |
4 D-glucose | - |
? | |
| 3.2.1.21 | gentiotriose + 2 H2O | Flavobacterium sp. | - |
3 D-glucose | - |
? | |
| 3.2.1.21 | luteose + H2O | Flavobacterium sp. | very low activity | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Flavobacterium sp. | - |
M64 | - |
| 3.2.1.21 | Flavobacterium sp. M64 | - |
M64 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography techniques | Flavobacterium sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Flavobacterium sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65.4 | - |
- |
Flavobacterium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | very low activity | Flavobacterium sp. | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Flavobacterium sp. | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | very low activity | Flavobacterium sp. M64 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Flavobacterium sp. M64 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Flavobacterium sp. | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Flavobacterium sp. M64 | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Flavobacterium sp. | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | transglycosylation reaction | Flavobacterium sp. | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Flavobacterium sp. M64 | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | transglycosylation reaction | Flavobacterium sp. M64 | beta-D-glucose + D-glucose | - |
r | |
| 3.2.1.21 | gentiobiose + H2O | - |
Flavobacterium sp. | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiopentaose + 4 H2O | specific for beta-1,6-linkages, activity decreases with number of glucose residues | Flavobacterium sp. | 5 D-glucose | - |
? | |
| 3.2.1.21 | gentiotetraose + 3 H2O | - |
Flavobacterium sp. | 4 D-glucose | - |
? | |
| 3.2.1.21 | gentiotriose + 2 H2O | - |
Flavobacterium sp. | 3 D-glucose | - |
? | |
| 3.2.1.21 | laminaran + H2O | low activity | Flavobacterium sp. | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | luteose + H2O | very low activity | Flavobacterium sp. | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | very low activity | Flavobacterium sp. | beta-D-glucose + methanol | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | - |
- |
Flavobacterium sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | - |
stable below | Flavobacterium sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.8 | - |
- |
Flavobacterium sp. |
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