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Literature summary extracted from

  • Glew, R.H.; Peters, S.P.; Christopher, A.R.
    Isolation and characterization of beta-glucosidase from the cytosol of rat kidney cortex (1976), Biochim. Biophys. Acta, 422, 179-199.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 2-mercaptoethanol 2fold enhanced activity Rattus sp.
3.2.1.21 L-cysteine 2.5fold enhanced activity at 10 mM Rattus sp.
3.2.1.21 Lubrol-WX 28% enhanced activity at 1%, w/v Rattus sp.
3.2.1.21 Tween-20 53% enhanced activity at 1%, w/v Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 5,5'-dithiobis(2-nitrobenzoic acid) nearly complete inhibition at 5 mM Rattus sp.
3.2.1.21 D-glucono-1,5-lactone Ki: 0.61 mM Rattus sp.
3.2.1.21 deoxycholic acid 89% inhibition at 1%, w/v Rattus sp.
3.2.1.21 Digitonin 70% inhibition at 1%, w/v Rattus sp.
3.2.1.21 DTT 28% inhibition at 10 mM Rattus sp.
3.2.1.21 GSH 50% inhibition at 7 mM Rattus sp.
3.2.1.21 iodoacetic acid strong inhibition at 5 mM Rattus sp.
3.2.1.21 lecithin 36% inhibition at 1%, w/v Rattus sp.
3.2.1.21 N-ethylmaleimide nearly complete inhibition at 5 mM Rattus sp.
3.2.1.21 taurocholic acid 74% inhibition at 1%, w/v Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.049
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rattus sp.
3.2.1.21 0.18
-
 4-methylumbelliferyl-beta-D-galactopyranoside
-
 Rattus sp.
3.2.1.21 0.26
-
 4-methylumbelliferyl-beta-D-glucopyranoside
-
 Rattus sp.
3.2.1.21 2.06
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rattus sp.
3.2.1.21 2.37
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cytosol
-
 Rattus sp. 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 50000 58000 gel filtration Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography techniques Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 renal cortex
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 1917
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-alpha-L-arabinoside + H2O
-
 Rattus sp. 4-methylumbelliferone + alpha-L-arabinose
-
 ?
3.2.1.21 4-methylumbelliferyl-beta-D-galactopyranoside + H2O
-
 Rattus sp. 4-methylumbelliferone + beta-D-galactopyranose
-
 ?
3.2.1.21 4-methylumbelliferyl-beta-D-glucoside + H2O
-
 Rattus sp. 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 50000-58000, SDS-PAGE Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6 7
-
 Rattus sp.