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Literature summary extracted from

  • Hirayama, T.; Horie, S.; Nagayama, H.; Matsuda, K.
    Studies on cellulases of a phytopathogenic fungus, Pyricularia oryzae Cavara. II. Purification and properties of a beta-glucosidase (1978), J. Biochem., 84, 27-37.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ nearly complete inhibition at 1 mM Pyricularia oryzae
3.2.1.21 D-glucono-1,5-lactone competitive inhibition, Ki: 0.007 mM Pyricularia oryzae
3.2.1.21 Hg2+ complete inhibition at 1 mM Pyricularia oryzae
3.2.1.21 Mn2+ 59% inhibition at 1 mM Pyricularia oryzae
3.2.1.21 p-chloromercuribenzoic acid strong inhibition at 1 mM Pyricularia oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.19
-
 laminaritetraose
-
 Pyricularia oryzae
3.2.1.21 0.26
-
 cellotetraose
-
 Pyricularia oryzae
3.2.1.21 0.34
-
 cellotriose
-
 Pyricularia oryzae
3.2.1.21 0.42
-
 laminaritriose
-
 Pyricularia oryzae
3.2.1.21 0.56
-
 gentiobiose
-
 Pyricularia oryzae
3.2.1.21 0.83
-
 laminaribiose
-
 Pyricularia oryzae
3.2.1.21 0.91
-
 cellobiose
-
 Pyricularia oryzae
3.2.1.21 1
-
 Salicin
-
 Pyricularia oryzae
3.2.1.21 1.33
-
 sophorose
-
 Pyricularia oryzae
3.2.1.21 1.43
-
 2-nitrophenyl-beta-D-glucoside
-
 Pyricularia oryzae
3.2.1.21 10
-
 methyl-beta-D-glucoside
-
 Pyricularia oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Pyricularia oryzae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 240000
-
 SDS-PAGE Pyricularia oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Pyricularia oryzae sophorose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Pyricularia oryzae laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Pyricularia oryzae cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Pyricularia oryzae gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Pyricularia oryzae
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Pyricularia oryzae
-
 3 D-glucose
-
 ?
3.2.1.21 laminaritetraose + 3 H2O Pyricularia oryzae
-
 4 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Pyricularia oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein
-
 Pyricularia oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography techniques, isoform GB-1 Pyricularia oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture 3 isoforms, isoform Gb-1 most strongly induced by growth in cellulose medium Pyricularia oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.04
-
-
 Pyricularia oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Pyricularia oryzae 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyricularia oryzae 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Pyricularia oryzae beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Pyricularia oryzae beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Pyricularia oryzae beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Pyricularia oryzae beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Pyricularia oryzae 2 beta-D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Pyricularia oryzae 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Pyricularia oryzae 3 D-glucose
-
 ?
3.2.1.21 CM-cellulose + H2O low activity Pyricularia oryzae beta-D-glucose + ?
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Pyricularia oryzae 2 beta-D-glucose
-
 ?
3.2.1.21 laminaran + H2O
-
 Pyricularia oryzae beta-D-glucose + ?
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Pyricularia oryzae 2 beta-D-glucose
-
 ?
3.2.1.21 laminaritetraose + 3 H2O
-
 Pyricularia oryzae 4 D-glucose
-
 ?
3.2.1.21 laminaritriose + H2O
-
 Pyricularia oryzae beta-D-glucose + laminaribiose
-
 ?
3.2.1.21 methyl-beta-D-glucoside + H2O
-
 Pyricularia oryzae beta-D-glucose + methanol
-
 ?
3.2.1.21 pustulan + H2O
-
 Pyricularia oryzae beta-D-glucose + ?
-
 ?
3.2.1.21 zeagallan + H2O
-
 Pyricularia oryzae beta-D-glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 55
-
-
 Pyricularia oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50
-
 stable up to Pyricularia oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
-
 Pyricularia oryzae