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Literature summary extracted from
- Fungal and other beta-D-glucosidases - their properties and applications (1982), Enzyme Microb. Technol., 4, 73-78.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | synthesis | increased hydrolysis of cellobiose by immobilized enzyme preparation | Prunus dulcis |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.21 | increased thermal stability by immobilization on concanavalin A-Sepharose, DEAE- or CM-Sephadex | Aspergillus niger |
| 3.2.1.21 | increased thermal stability by immobilization on concanavalin A-Sepharose, DEAE- or CM-Sephadex | Prunus dulcis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | beta-D-glucose | - |
Acetivibrio thermocellus |  |
| 3.2.1.21 | beta-D-glucose | - |
Aspergillus niger | |
| 3.2.1.21 | beta-D-glucose | - |
Aspergillus oryzae | |
| 3.2.1.21 | beta-D-glucose | - |
Aspergillus wentii | |
| 3.2.1.21 | beta-D-glucose | - |
Homo sapiens | |
| 3.2.1.21 | beta-D-glucose | - |
Malus domestica | |
| 3.2.1.21 | beta-D-glucose | - |
Pantoea agglomerans | |
| 3.2.1.21 | beta-D-glucose | - |
Prunus dulcis | |
| 3.2.1.21 | beta-D-glucose | - |
Pyrus communis | |
| 3.2.1.21 | beta-D-glucose | - |
Rhizomucor miehei | |
| 3.2.1.21 | beta-D-glucose | - |
Saccharomyces cerevisiae | |
| 3.2.1.21 | beta-D-glucose | - |
Trichoderma reesei | |
| 3.2.1.21 | conduritol-B-epoxide | - |
Aspergillus wentii | |
| 3.2.1.21 | conduritol-B-epoxide | bound as an ester of (+) chiro-inositol to aspartic acid | Prunus dulcis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.095 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Saccharomyces cerevisiae | |
| 3.2.1.21 | 0.58 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Rhizomucor miehei | |
| 3.2.1.21 | 0.72 | - |
Salicin | - |
Rhizomucor miehei | |
| 3.2.1.21 | 1.04 | - |
cellobiose | - |
Rhizomucor miehei | |
| 3.2.1.21 | 2.1 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Acetivibrio thermocellus | |
| 3.2.1.21 | 83 | - |
cellobiose | - |
Acetivibrio thermocellus | |
| 3.2.1.21 | 117 | - |
cellobiose | increased Km if enzyme immobilized on cellulose | Prunus dulcis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Aspergillus niger | - |
- |
| 3.2.1.21 | extracellular | - |
Aspergillus oryzae | - |
- |
| 3.2.1.21 | extracellular | - |
Trichoderma reesei | - |
- |
| 3.2.1.21 | extracellular | - |
Rhizomucor miehei | - |
- |
| 3.2.1.21 | extracellular | - |
Aspergillus wentii | - |
- |
| 3.2.1.21 | intracellular | - |
Saccharomyces cerevisiae | 5622 | - |
| 3.2.1.21 | lysosome | - |
Homo sapiens | 5764 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50000 | - |
- |
Acetivibrio thermocellus |
| 3.2.1.21 | 122000 | - |
- |
Pantoea agglomerans |
| 3.2.1.21 | 218000 | - |
- |
Aspergillus oryzae |
| 3.2.1.21 | 300000 | - |
yeast foam strain | Saccharomyces cerevisiae |
| 3.2.1.21 | 313000 | - |
intracellular enzyme | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Homo sapiens | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Saccharomyces cerevisiae | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus niger | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Pantoea agglomerans | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Prunus dulcis | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus oryzae | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Trichoderma reesei | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Rhizomucor miehei | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Malus domestica | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Pyrus communis | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Acetivibrio thermocellus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus wentii | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | important in processing of oligosaccharides after their transfer from dolichyl diphosphate to proteins during glycoprotein synthesis | Glc2Man9GlcNAc2 + beta-D-glucose | - |
? | |
| 3.2.1.21 | glucosyl ceramide + H2O | Homo sapiens | involved in normal catabolism of glucosyl ceramides | sphingosine + fatty acid + beta-D-glucose | - |
? | |
| 3.2.1.21 | n-decyl beta-D-glucoside + H2O | Saccharomyces cerevisiae | 6fold higher activity than with methyl-beta-D-glucoside | beta-D-glucose + n-decanol | - |
? | |
| 3.2.1.21 | starch + H2O | Aspergillus niger | - |
beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Acetivibrio thermocellus | - |
- |
- |
| 3.2.1.21 | Aspergillus niger | - |
- |
- |
| 3.2.1.21 | Aspergillus oryzae | - |
- |
- |
| 3.2.1.21 | Aspergillus wentii | - |
- |
- |
| 3.2.1.21 | Homo sapiens | - |
- |
- |
| 3.2.1.21 | Malus domestica | - |
- |
- |
| 3.2.1.21 | Pantoea agglomerans | - |
- |
- |
| 3.2.1.21 | Prunus dulcis | - |
var. sativa | - |
| 3.2.1.21 | Pyrus communis | - |
- |
- |
| 3.2.1.21 | Rhizomucor miehei | - |
- |
- |
| 3.2.1.21 | Saccharomyces cerevisiae | - |
- |
- |
| 3.2.1.21 | Trichoderma reesei | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Aspergillus niger |
| 3.2.1.21 | glycoprotein | - |
Trichoderma reesei |
| 3.2.1.21 | glycoprotein | 23.4% carbohydrate content, w/w, enzymatic removal of carbohydrate moiety decreases thermal stability | Rhizomucor miehei |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Aspergillus niger | - |
| 3.2.1.21 | cell culture | - |
Aspergillus oryzae | - |
| 3.2.1.21 | cell culture | - |
Rhizomucor miehei | - |
| 3.2.1.21 | cell culture | - |
Acetivibrio thermocellus | - |
| 3.2.1.21 | cell culture | - |
Aspergillus wentii | - |
| 3.2.1.21 | cell culture | potent formation of cellobiase activity by induction by sophorose | Trichoderma reesei | - |
| 3.2.1.21 | reticuloendothelial system | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Aspergillus niger | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Trichoderma reesei | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Malus domestica | 4-hydroxyphenol + beta-D-glucopyranose | product 4-hydroxyphenol toxic to invading pathogens | ? | |
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Pyrus communis | 4-hydroxyphenol + beta-D-glucopyranose | product 4-hydroxyphenol toxic to invading pathogens | ? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pantoea agglomerans | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Prunus dulcis | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Malus domestica | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrus communis | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus wentii | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | higher activity than on cellobiose | Saccharomyces cerevisiae | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | less active than on cellobiose | Aspergillus oryzae | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Prunus dulcis | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Aspergillus niger | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Trichoderma reesei | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Acetivibrio thermocellus | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Homo sapiens | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Saccharomyces cerevisiae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus niger | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Pantoea agglomerans | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Prunus dulcis | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus oryzae | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Rhizomucor miehei | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Malus domestica | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Pyrus communis | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Acetivibrio thermocellus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus wentii | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Rhizomucor miehei | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Acetivibrio thermocellus | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | Glc3Man9GlcNAc2 + H2O | important in processing of oligosaccharides after their transfer from dolichyl diphosphate to proteins during glycoprotein synthesis | Saccharomyces cerevisiae | Glc2Man9GlcNAc2 + beta-D-glucose | - |
? | |
| 3.2.1.21 | glucosyl ceramide + H2O | involved in normal catabolism of glucosyl ceramides | Homo sapiens | sphingosine + fatty acid + beta-D-glucose | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Homo sapiens | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Aspergillus niger | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Pantoea agglomerans | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Prunus dulcis | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Aspergillus oryzae | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Trichoderma reesei | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Rhizomucor miehei | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Malus domestica | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Pyrus communis | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | - |
Acetivibrio thermocellus | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | n-decyl beta-D-glucoside + H2O | 6fold higher activity than with methyl-beta-D-glucoside | Saccharomyces cerevisiae | beta-D-glucose + n-decanol | - |
? | |
| 3.2.1.21 | salicin + H2O | - |
Rhizomucor miehei | D-glucose + salicyl alcohol | - |
? | |
| 3.2.1.21 | starch + H2O | - |
Aspergillus niger | beta-D-glucose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 45 | - |
- |
Saccharomyces cerevisiae |
| 3.2.1.21 | 45 | - |
yeast foam strain | Malus domestica |
| 3.2.1.21 | 65 | - |
- |
Trichoderma reesei |
| 3.2.1.21 | 65 | - |
- |
Rhizomucor miehei |
| 3.2.1.21 | 65 | - |
- |
Acetivibrio thermocellus |
| 3.2.1.21 | 65 | - |
- |
Aspergillus wentii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 45 | - |
- |
Aspergillus oryzae |
| 3.2.1.21 | 45 | - |
yeast foam strain | Saccharomyces cerevisiae |
| 3.2.1.21 | 50 | - |
- |
Homo sapiens |
| 3.2.1.21 | 50 | - |
- |
Pantoea agglomerans |
| 3.2.1.21 | 60 | - |
- |
Aspergillus niger |
| 3.2.1.21 | 60 | - |
- |
Trichoderma reesei |
| 3.2.1.21 | 60 | - |
- |
Acetivibrio thermocellus |
| 3.2.1.21 | 60 | - |
- |
Aspergillus wentii |
| 3.2.1.21 | 70 | - |
stable up to | Rhizomucor miehei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 5 | - |
Aspergillus oryzae |
| 3.2.1.21 | 5.6 | - |
- |
Prunus dulcis |
| 3.2.1.21 | 6 | 7.5 | - |
Pantoea agglomerans |
| 3.2.1.21 | 6 | - |
- |
Acetivibrio thermocellus |
| 3.2.1.21 | 6.4 | 6.8 | yeast foam strain | Saccharomyces cerevisiae |
| 3.2.1.21 | 6.8 | - |
intracellular enzyme | Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | 10 | highly active between | Rhizomucor miehei |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | 7 | - |
Aspergillus oryzae |
| 3.2.1.21 | 6 | 8 | yeast foam strain | Saccharomyces cerevisiae |
| 3.2.1.21 | 6 | 7.5 | - |
Pantoea agglomerans |
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