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Literature summary extracted from

  • Santos, C.D.; Terra, W.R.
    Physical properties, substrate specificities and a probable mechanism for a beta-D-glucosidase (cellobiase) from midgut cells of the cassava hornworm (Erinnyis ello) (1985), Biochim. Biophys. Acta, 831, 179-185.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 D-glucono-1,5-lactone competitive inhibition, Ki: 0.00085 mM Erinnyis ello
3.2.1.21 gamma-galactonolactone competitive inhibition, Ki: 0.16 mM Erinnyis ello
3.2.1.21 Tris competitive inhibition, Ki: 0.54 mM Erinnyis ello

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.18
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Erinnyis ello
3.2.1.21 0.33
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Erinnyis ello
3.2.1.21 0.33
-
 4-nitrophenyl-beta-D-fucoside
-
 Erinnyis ello
3.2.1.21 0.63
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Erinnyis ello
3.2.1.21 1
-
 4-nitrophenyl-beta-D-xyloside
-
 Erinnyis ello
3.2.1.21 2
-
 cellobiose
-
 Erinnyis ello
3.2.1.21 6.3
-
 Salicin
-
 Erinnyis ello
3.2.1.21 7.4
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Erinnyis ello
3.2.1.21 17
-
 lactose
-
 Erinnyis ello

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 129000
-
 density gradient centrifugation, isoelectric focusing Erinnyis ello

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Erinnyis ello lactose beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Erinnyis ello cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Erinnyis ello
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 midgut from larvae Erinnyis ello
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Erinnyis ello 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Erinnyis ello 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O retention of configuration Erinnyis ello 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
 Erinnyis ello 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Erinnyis ello 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Erinnyis ello 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Erinnyis ello 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Erinnyis ello beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Erinnyis ello beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Erinnyis ello 2 beta-D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 53
-
 inactivation above Erinnyis ello

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5
-
-
 Erinnyis ello

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 6.5
-
 stable for 2 h at 15-40°C Erinnyis ello