Any feedback?
Literature summary extracted from
- Purification and properties of a stable beta-glucosidase from an extremely thermophilic anaerobic bacterium (1987), Biochem. J., 243, 779-787.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | 2-mercaptoethanol | 30% stimulation at 1 mM | thermophilic anaerobic bacterium |  |
| 3.2.1.21 | Cysteine hydrochloride | slight activation at 5 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | dithiothreitol | slight activation at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | EDTA | slight activation at 5 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | glycerol | 25% stimulation at 10%, w/v | thermophilic anaerobic bacterium | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.21 | glycerol most effective in stabilizing | thermophilic anaerobic bacterium |
| 3.2.1.21 | sensitive against repeated thawing and freezing | thermophilic anaerobic bacterium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Ag+ | complete inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Al3+ | strong inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Ba2+ | slight inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Ca2+ | slight inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Cd2+ | strong inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Co2+ | nearly complete inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Cu2+ | complete inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | ethanol | 20% inhibition at 5%, w/v | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Hg2+ | complete inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Mn2+ | slight inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Ni2+ | strong inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | p-chloromercuribenzene sulfonic acid | nearly complete inhibition at 1 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | SDS | 87% inhibition at 1%, w/v | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Tris | inhibitory effect | thermophilic anaerobic bacterium | |
| 3.2.1.21 | Triton X-100 | 25% inhibition at 1%, w/v | thermophilic anaerobic bacterium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.15 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
thermophilic anaerobic bacterium | |
| 3.2.1.21 | 0.73 | - |
cellobiose | - |
thermophilic anaerobic bacterium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | cell associated | - |
thermophilic anaerobic bacterium | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Br- | slight activation at 5 mM | thermophilic anaerobic bacterium | |
| 3.2.1.21 | I- | 40% activation at 5 mM | thermophilic anaerobic bacterium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 43000 | 50000 | gel filtration, SDS-PAGE | thermophilic anaerobic bacterium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | thermophilic anaerobic bacterium | sophorose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | thermophilic anaerobic bacterium | lactose | beta-D-galactose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | thermophilic anaerobic bacterium | cellobiose | beta-D-glucose + D-glucose | additional product: cellotriose by transglycosylation activity | r | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | thermophilic anaerobic bacterium | transglycolsylation reaction, yielding alcohol-beta-glucosides in the presence of alcohols | beta-D-glucose + D-glucose | additional product: cellotriose by transglycosylation activity | r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | thermophilic anaerobic bacterium | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | thermophilic anaerobic bacterium | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | laminarin + H2O | thermophilic anaerobic bacterium | low activity | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | thermophilic anaerobic bacterium | - |
thermophilic anaerobic gram-negative bacterium | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
thermophilic anaerobic bacterium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography steps, preparative gel electrophoresis | thermophilic anaerobic bacterium |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
thermophilic anaerobic bacterium | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 149 | - |
- |
thermophilic anaerobic bacterium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.21 | 4°C, 1-2% loss of activity per week | thermophilic anaerobic bacterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | thermophilic anaerobic bacterium | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | thermophilic anaerobic bacterium | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
thermophilic anaerobic bacterium | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
thermophilic anaerobic bacterium | 2-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | thermophilic anaerobic bacterium | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | thermophilic anaerobic bacterium | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | thermophilic anaerobic bacterium | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | thermophilic anaerobic bacterium | beta-D-galactose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | thermophilic anaerobic bacterium | beta-D-glucose + D-glucose | additional product: cellotriose by transglycosylation activity | r | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | transglycolsylation reaction, yielding alcohol-beta-glucosides in the presence of alcohols | thermophilic anaerobic bacterium | beta-D-glucose + D-glucose | additional product: cellotriose by transglycosylation activity | r | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | thermophilic anaerobic bacterium | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | thermophilic anaerobic bacterium | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
thermophilic anaerobic bacterium | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | esculin + H2O | - |
thermophilic anaerobic bacterium | beta-D-glucose + 6,7-dihydroxycoumarin | - |
? | |
| 3.2.1.21 | laminarin + H2O | low activity | thermophilic anaerobic bacterium | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | phenyl-beta-D-glucoside + H2O | low activity | thermophilic anaerobic bacterium | phenol + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | monomer | 1 * 43000-50000, SDS-PAGE | thermophilic anaerobic bacterium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 75 | - |
half-life: 130 min at pH 7.0 | thermophilic anaerobic bacterium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6.2 | - |
- |
thermophilic anaerobic bacterium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
