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Literature summary extracted from

  • Plant, A.R.; Oliver, J.E.; Patchett, M.L.; Daniel, R.M.; Morgan, H.W.
    Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli (1988), Arch. Biochem. Biophys., 262, 181-188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Caldicellulosiruptor saccharolyticus

General Stability

EC Number General Stability Organism
3.2.1.21 stable in the presence of cetyl-trimethylammonium bromide Caldicellulosiruptor saccharolyticus
3.2.1.21 stable in the presence of urea Caldicellulosiruptor saccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 1-O-methyl-beta-D-glucose competitive inhibition, Ki: 17.5 mM Caldicellulosiruptor saccharolyticus
3.2.1.21 Co2+ destabilizing effect Caldicellulosiruptor saccharolyticus
3.2.1.21 D-glucono-1,4-lactone Ki: 1.6 mM Caldicellulosiruptor saccharolyticus
3.2.1.21 D-glucono-1,5-lactone Ki: 0.09 mM Caldicellulosiruptor saccharolyticus
3.2.1.21 Hg2+ strong inactivation at 0.5 mM, reversal by addition of 10 mM beta-mercaptoethanol Caldicellulosiruptor saccharolyticus
3.2.1.21 La3+ rapid inactivation at 3 mM Caldicellulosiruptor saccharolyticus
3.2.1.21 N-ethylmaleimide rapid loss of activity at 5 mM, reversal by addition of beta-mercaptoethanol Caldicellulosiruptor saccharolyticus
3.2.1.21 Ni2+ strong inhibition Caldicellulosiruptor saccharolyticus
3.2.1.21 SDS complete inhibition at 1 mg/ml Caldicellulosiruptor saccharolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.24
-
4-nitrophenyl-beta-D-fucoside
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 0.29
-
2-nitrophenyl beta-D-glucopyranoside
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 0.51
-
4-nitrophenyl-beta-D-glucopyranoside
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 1.88
-
Salicin
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 1.94
-
4-nitrophenyl-beta-D-galactopyranoside
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 2.12
-
2-nitrophenyl beta-D-galactopyranoside
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 3
-
laminaribiose
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 5.2
-
sophorose
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 6.93
-
4-nitrophenyl-beta-D-xyloside
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 18.2
-
cellobiose
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 21.5
-
gentiobiose
-
Caldicellulosiruptor saccharolyticus
3.2.1.21 120
-
lactose
-
Caldicellulosiruptor saccharolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Caldicellulosiruptor saccharolyticus sophorose beta-D-glucose + D-glucose
-
?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Caldicellulosiruptor saccharolyticus laminaribiose beta-D-glucose + D-glucose
-
?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Caldicellulosiruptor saccharolyticus lactose beta-D-galactose + D-glucose
-
?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Caldicellulosiruptor saccharolyticus cellobiose beta-D-glucose + D-glucose
-
?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Caldicellulosiruptor saccharolyticus gentiobiose beta-D-glucose + D-glucose
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.21 tetrahydrofuran unstable above 20%, v/v Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Caldicellulosiruptor saccharolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, recombinant enzyme Caldicellulosiruptor saccharolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
Caldicellulosiruptor saccharolyticus
-
3.2.1.21 additional information
-
Caldicellulosiruptor saccharolyticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 560
-
-
Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Caldicellulosiruptor saccharolyticus 2-hydroxymethylphenol + beta-D-glucose
-
?
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
Caldicellulosiruptor saccharolyticus 2-nitrophenol + beta-D-galactopyranose
-
?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
Caldicellulosiruptor saccharolyticus 2-nitrophenol + beta-D-glucopyranose
-
?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Caldicellulosiruptor saccharolyticus beta-D-glucose + D-glucose
-
?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Caldicellulosiruptor saccharolyticus beta-D-glucose + D-glucose
-
?
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-fucose
-
?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-galactose
-
?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Caldicellulosiruptor saccharolyticus 4-nitrophenol + D-glucopyranose
-
?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-xylose
-
?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Caldicellulosiruptor saccharolyticus beta-D-galactose + D-glucose
-
?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Caldicellulosiruptor saccharolyticus beta-D-glucose + D-glucose
-
?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Caldicellulosiruptor saccharolyticus beta-D-glucose + D-glucose
-
?
3.2.1.21 cellobiose + H2O
-
Caldicellulosiruptor saccharolyticus 2 beta-D-glucose
-
?
3.2.1.21 gentiobiose + H2O
-
Caldicellulosiruptor saccharolyticus 2 beta-D-glucose
-
?
3.2.1.21 laminaribiose + H2O
-
Caldicellulosiruptor saccharolyticus 2 beta-D-glucose
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 80
-
half-life: 15 h Caldicellulosiruptor saccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6 6.5
-
Caldicellulosiruptor saccharolyticus