EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | expression in Escherichia coli | Caldicellulosiruptor saccharolyticus |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.21 | stable in the presence of cetyl-trimethylammonium bromide | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | stable in the presence of urea | Caldicellulosiruptor saccharolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 1-O-methyl-beta-D-glucose | competitive inhibition, Ki: 17.5 mM | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | Co2+ | destabilizing effect | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | D-glucono-1,4-lactone | Ki: 1.6 mM | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | D-glucono-1,5-lactone | Ki: 0.09 mM | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | Hg2+ | strong inactivation at 0.5 mM, reversal by addition of 10 mM beta-mercaptoethanol | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | La3+ | rapid inactivation at 3 mM | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | N-ethylmaleimide | rapid loss of activity at 5 mM, reversal by addition of beta-mercaptoethanol | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | Ni2+ | strong inhibition | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | SDS | complete inhibition at 1 mg/ml | Caldicellulosiruptor saccharolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.24 | - |
4-nitrophenyl-beta-D-fucoside | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 0.29 | - |
2-nitrophenyl beta-D-glucopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 0.51 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 1.88 | - |
Salicin | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 1.94 | - |
4-nitrophenyl-beta-D-galactopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 2.12 | - |
2-nitrophenyl beta-D-galactopyranoside | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 3 | - |
laminaribiose | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 5.2 | - |
sophorose | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 6.93 | - |
4-nitrophenyl-beta-D-xyloside | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 18.2 | - |
cellobiose | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 21.5 | - |
gentiobiose | - |
Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 120 | - |
lactose | - |
Caldicellulosiruptor saccharolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Caldicellulosiruptor saccharolyticus | sophorose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Caldicellulosiruptor saccharolyticus | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Caldicellulosiruptor saccharolyticus | lactose | beta-D-galactose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Caldicellulosiruptor saccharolyticus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Caldicellulosiruptor saccharolyticus | gentiobiose | beta-D-glucose + D-glucose | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.1.21 | tetrahydrofuran | unstable above 20%, v/v | Caldicellulosiruptor saccharolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Caldicellulosiruptor saccharolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | to homogeneity, recombinant enzyme | Caldicellulosiruptor saccharolyticus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | cell culture | - |
Caldicellulosiruptor saccharolyticus | - |
3.2.1.21 | additional information | - |
Caldicellulosiruptor saccharolyticus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 560 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Caldicellulosiruptor saccharolyticus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-fucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-xylose | - |
? | |
3.2.1.21 | 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Caldicellulosiruptor saccharolyticus | beta-D-galactose + D-glucose | - |
? | |
3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Caldicellulosiruptor saccharolyticus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? | |
3.2.1.21 | gentiobiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? | |
3.2.1.21 | laminaribiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 80 | - |
half-life: 15 h | Caldicellulosiruptor saccharolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 6 | 6.5 | - |
Caldicellulosiruptor saccharolyticus |