Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bronnenmeier, K.; Staudenbauer, W.L.
    Purification and properties of an extracellular beta-glucosidase from the cellulolytic thermophile Clostridium stercorarium (1988), Appl. Microbiol. Biotechnol., 28, 380-386.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 dithiothreitol stimulation at 10 mM Thermoclostridium stercorarium

General Stability

EC Number General Stability Organism
3.2.1.21 stabilizing effect by divalent cations Thermoclostridium stercorarium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Hg2+ strong inhibition at 1 mM Thermoclostridium stercorarium
3.2.1.21 p-hydroxymercuribenzoate strong inhibition at 1 mM Thermoclostridium stercorarium
3.2.1.21 SDS complete inhibition at 0.05%, w/v Thermoclostridium stercorarium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.8
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Thermoclostridium stercorarium
3.2.1.21 33
-
 cellobiose
-
 Thermoclostridium stercorarium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Thermoclostridium stercorarium
-
-
3.2.1.21 periplasm
-
 Thermoclostridium stercorarium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ stimulation at 10 mM Thermoclostridium stercorarium
3.2.1.21 Mg2+ stimulation at 10 mM Thermoclostridium stercorarium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 85000
-
 1 * 85000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.21 85000 105000 gel filtration, SDS-PAGE Thermoclostridium stercorarium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermoclostridium stercorarium laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermoclostridium stercorarium cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellulose + H2O Thermoclostridium stercorarium cellulose MN 300 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermoclostridium stercorarium
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.1.21 addition of DTT increases thermostability Thermoclostridium stercorarium

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, extracellular form, 2step chromatography Thermoclostridium stercorarium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture two forms, one membrane-bound, one extracellular Thermoclostridium stercorarium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 31.6
-
 extracellular beta-glucosidase Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Thermoclostridium stercorarium 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Thermoclostridium stercorarium 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Thermoclostridium stercorarium beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-methylumbelliferyl-beta-D-glucoside + H2O
-
 Thermoclostridium stercorarium 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O quite specific for beta-1,4-linkages Thermoclostridium stercorarium 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O low activity Thermoclostridium stercorarium 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermoclostridium stercorarium beta-D-glucose + D-glucose
-
 ?
3.2.1.21 Avicel + H2O acid-swollen, low activity Thermoclostridium stercorarium beta-D-glucose + ?
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermoclostridium stercorarium 2 beta-D-glucose
-
 ?
3.2.1.21 cellulose + H2O cellulose MN 300 Thermoclostridium stercorarium beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 85000, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 65
-
-
 Thermoclostridium stercorarium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 75
-
 sharp decline of activity above, increase thermostability by addition of DTT and/or divalent cations Thermoclostridium stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
-
 Thermoclostridium stercorarium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5 7 optimal activity within Thermoclostridium stercorarium