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Literature summary extracted from
- Construction of chimeric beta-glucosidases with improved enzymatic properties (1995), J. Biol. Chem., 270, 21928-21933.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expression in Escherichia coli | Agrobacterium tumefaciens |
| 3.2.1.21 | expression in Escherichia coli | Cellulomonas gilvus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.005 | - |
4-nitrophenyl-beta-D-xyloside | no change for chimeric enzymes | Agrobacterium tumefaciens |  |
| 3.2.1.21 | 0.032 | - |
4-nitrophenyl-beta-D-glucopyranoside | marked increase for chimeric enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 0.12 | - |
4-nitrophenyl-beta-D-fucoside | - |
Agrobacterium tumefaciens | |
| 3.2.1.21 | 1.8 | - |
4-nitrophenyl-beta-D-glucopyranoside | marked decrease for chimeric enzymes | Cellulomonas gilvus | |
| 3.2.1.21 | 6.3 | - |
4-nitrophenyl-beta-D-xyloside | - |
Cellulomonas gilvus | |
| 3.2.1.21 | 10.8 | - |
4-nitrophenyl-beta-D-galactoside | - |
Cellulomonas gilvus | |
| 3.2.1.21 | 13.1 | - |
4-nitrophenyl-beta-D-galactoside | - |
Agrobacterium tumefaciens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Agrobacterium tumefaciens | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Cellulomonas gilvus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | Agrobacterium tumefaciens | marked increase of relative activity for chimeric enzymes | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | Cellulomonas gilvus | marked increase of relative activity for chimeric enzymes | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | Agrobacterium tumefaciens | marked increase of relative activity for chimeric enzyme | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | Cellulomonas gilvus | marked increase of relative activity for chimeric enzyme | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | Agrobacterium tumefaciens | marked increase of relative activity for chimeric activity | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | Cellulomonas gilvus | marked increase of relative activity for chimeric activity | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | Agrobacterium tumefaciens | - |
3 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | Cellulomonas gilvus | - |
3 D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Agrobacterium tumefaciens | - |
- |
- |
| 3.2.1.21 | Cellulomonas gilvus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | partial | Agrobacterium tumefaciens |
| 3.2.1.21 | partial | Cellulomonas gilvus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Agrobacterium tumefaciens | - |
| 3.2.1.21 | cell culture | - |
Cellulomonas gilvus | - |
| 3.2.1.21 | additional information | genomic library | Agrobacterium tumefaciens | - |
| 3.2.1.21 | additional information | genomic library | Cellulomonas gilvus | - |
| 3.2.1.21 | additional information | construction of 3 chimeric enzymes derived from substituting segments from the C-terminus of Cellvibrio gilvus and Agrobacterium tumefaciens beta-glucosidase genes | Agrobacterium tumefaciens | - |
| 3.2.1.21 | additional information | construction of 3 chimeric enzymes derived from substituting segments from the C-terminus of Cellvibrio gilvus and Agrobacterium tumefaciens beta-glucosidase genes | Cellulomonas gilvus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Agrobacterium tumefaciens | 4-methylumbelliferone + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Cellulomonas gilvus | 4-methylumbelliferone + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Cellulomonas gilvus | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Cellulomonas gilvus | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas gilvus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellulomonas gilvus | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Agrobacterium tumefaciens | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Cellulomonas gilvus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | marked increase of relative activity for chimeric enzymes | Agrobacterium tumefaciens | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | marked increase of relative activity for chimeric enzymes | Cellulomonas gilvus | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | marked increase of relative activity for chimeric enzyme | Agrobacterium tumefaciens | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | marked increase of relative activity for chimeric enzyme | Cellulomonas gilvus | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | marked increase of relative activity for chimeric activity | Agrobacterium tumefaciens | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | marked increase of relative activity for chimeric activity | Cellulomonas gilvus | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | - |
Agrobacterium tumefaciens | 3 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | - |
Cellulomonas gilvus | 3 D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 35 | - |
- |
Cellulomonas gilvus |
| 3.2.1.21 | 45 | 50 | for chimeric enzymes | Agrobacterium tumefaciens |
| 3.2.1.21 | 45 | 50 | for chimeric enzymes | Cellulomonas gilvus |
| 3.2.1.21 | 60 | - |
- |
Agrobacterium tumefaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6.2 | 6.4 | - |
Cellulomonas gilvus |
| 3.2.1.21 | 6.6 | 7 | for chimeric enzymes | Agrobacterium tumefaciens |
| 3.2.1.21 | 6.6 | 7 | for chimeric enzymes | Cellulomonas gilvus |
| 3.2.1.21 | 7.2 | 7.4 | - |
Agrobacterium tumefaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 9 | chimeric enzymes, stable within | Agrobacterium tumefaciens |
| 3.2.1.21 | 4 | 9 | chimeric enzymes, stable within | Cellulomonas gilvus |
| 3.2.1.21 | 4 | 8 | stable within | Cellulomonas gilvus |
| 3.2.1.21 | 5 | 10 | stable within | Agrobacterium tumefaciens |
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