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Literature summary extracted from

  • Bobkova, E.V.; Stepanov, V.G.; Lavrik, O.I.
    A comparative study of the relationship between thermostability and function of phenylalanyl-tRNA synthetase from Escherichia coli and Thermus thermophilus (1992), FEBS Lett., 302, 54-56.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Escherichia coli
-
-
-
6.1.1.20 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.20 43
-
ATP-diphosphate exchange Escherichia coli
6.1.1.20 85
-
ATP-diphosphate exchange Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.20 44
-
about 50% loss of activity after 50 min Escherichia coli
6.1.1.20 90
-
about 30% loss of activity after 80 min Thermus thermophilus
6.1.1.20 95
-
about 45% loss of activity after 20 min Thermus thermophilus