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Literature summary extracted from

  • Yorifuji, T.; Sugai, I.; Matsumoto, H.; Tabuchi, A.
    Characterization of 3-guanidinopropionate amidinohydrolase from Pseudomonas aeruginosa and a comparative study with 4-guanidinobutyrate amidinohydrolase from another Pseudomonas (1982), Agric. Biol. Chem., 46, 1361-1367.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.7 3-Amino-1-propanol 5 mM, relative activity 113% Pseudomonas sp.
3.5.3.7 DL-lactate 5 mM, relative activity 106% Pseudomonas sp.
3.5.3.7 fumarate 5 mM, relative activity 101% Pseudomonas sp.
3.5.3.7 Guanidinoacetate 5 mM, relative activity 104% Pseudomonas sp.
3.5.3.7 Maleate 5 mM, relative activity 104% Pseudomonas sp.
3.5.3.7 n-butylamine 5 mM, relative activity 118% Pseudomonas sp.
3.5.3.7 n-Propylamine 5 mM, relative activity 121% Pseudomonas sp.
3.5.3.7 succinate 5 mM, relative activity 103% Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.7
-
 Pseudomonas sp.
3.5.3.17
-
 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.7 4-aminobutyrate
-
 Pseudomonas sp.
3.5.3.7 n-Butyrate competitive inhibition Pseudomonas sp.
3.5.3.7 propionate competitive inhibition Pseudomonas sp.
3.5.3.7 trans-crotonate
-
 Pseudomonas sp.
3.5.3.17 4-aminobutyrate
-
 Pseudomonas aeruginosa
3.5.3.17 EDTA reactivation by Mn2+ Pseudomonas aeruginosa
3.5.3.17 n-Butyrate
-
 Pseudomonas aeruginosa
3.5.3.17 PCMB reactivation by 2-mercaptoethanol Pseudomonas aeruginosa
3.5.3.17 propionate
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.17 45
-
 3-guanidinopropionate
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.7 Mn2+
-
 Pseudomonas fluorescens
3.5.3.7 Mn2+
-
 Pseudomonas putida
3.5.3.7 Mn2+ absolute requirement Pseudomonas sp.
3.5.3.17 Mn2+ purified native enzyme is fully active without added divalent metal, most potent activator of EDTA-treated enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 36000
-
 6 * 36000, SDS-PAGE Pseudomonas sp.
3.5.3.7 178000
-
-
 Pseudomonas putida
3.5.3.7 180000 186000
-
 Pseudomonas sp.
3.5.3.7 190000
-
-
 Pseudomonas putida
3.5.3.17 36000
-
 x * 36000, Pseudomonas aeruginosa, SDS-PAGE Pseudomonas aeruginosa
3.5.3.17 195000 215000 Pseudomonas aeruginosa, gel filtration of crystalline enzyme, disc gel electrophoresis Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas fluorescens
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas sp.
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas putida
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas sp. ATCC 14676
-
 4-Aminobutyrate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Pseudomonas fluorescens
-
-
-
3.5.3.7 Pseudomonas putida
-
 P2 (ATCC 25571)
-
3.5.3.7 Pseudomonas sp.
-
-
-
3.5.3.7 Pseudomonas sp. ATCC 14676
-
-
-
3.5.3.17 Pseudomonas aeruginosa
-
-
-
3.5.3.17 Pseudomonas aeruginosa
-
 ATCC 15692
-
3.5.3.17 Pseudomonas aeruginosa PAO 1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Pseudomonas fluorescens
3.5.3.7
-
 Pseudomonas sp.
3.5.3.7
-
 Pseudomonas putida
3.5.3.17
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.17 additional information
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.5.3.17 -20°C, 10 mM Tris-chloride buffer, pH 8.0, 0.05 mM MnCl2, 25% glycerol, partially purified enzyme is stable for up to 5 months Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas fluorescens 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas putida 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas sp. ATCC 14676 4-Aminobutyrate + urea
-
 ?
3.5.3.17 3-Guanidinopropanoate + H2O
-
 Pseudomonas aeruginosa beta-Alanine + urea
-
 ?
3.5.3.17 3-Guanidinopropanoate + H2O
-
 Pseudomonas aeruginosa PAO 1 beta-Alanine + urea
-
 ?
3.5.3.17 4-Guanidinobutyrate + H2O 3% of the activity with 3-guanidinopropanoate Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
 ?
3.5.3.17 4-Guanidinobutyrate + H2O 3% of the activity with 3-guanidinopropanoate Pseudomonas aeruginosa PAO 1 4-Aminobutyrate + urea
-
 ?
3.5.3.17 Taurocyamine + H2O 11% of the activity with 3-guanidinopropanoate Pseudomonas aeruginosa ?
-
 ?
3.5.3.17 Taurocyamine + H2O 11% of the activity with 3-guanidinopropanoate Pseudomonas aeruginosa PAO 1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.7 hexamer 6 * 36000, SDS-PAGE Pseudomonas sp.
3.5.3.17 ? x * 36000, Pseudomonas aeruginosa, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 10
-
-
 Pseudomonas sp.
3.5.3.7 10
-
-
 Pseudomonas putida
3.5.3.17 9
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.17 8 9.5 30% of activity maximum at pH 8.0 and 9.5 Pseudomonas aeruginosa