BRENDA - Enzyme Database show

Immunoaffinity purification of human thromboxane synthase

Nusing, R.; Schneider-Voss, S.; Ullrich, V.; Arch. Biochem. Biophys. 280, 325-330 (1990)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.01
-
prostaglandin H2
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.5
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.5
58000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.5
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.5
one-step immunoaffinity purification
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.5
platelet
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.5
0.255
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
Prostaglandin H2
i.e. PGH2
3194
Homo sapiens
Thromboxane B2 + 12(L)-hydroxy-5,8,10-heptadecatrienoic acid
the ratio of thromboxane to C17 hydroxyfatty acid formation is 1:1
3194
Homo sapiens
-
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.5
?
-
Homo sapiens
5.3.99.5
monomer
x * 58000, SDS-PAGE
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.5
heme
contains one heme per 58000 MW protein. The major part of the enzyme is converted by the elution procedure to the inactive P420 form, a minor part is in its native heme-thiolate conformation; hemoprotein
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.5
heme
contains one heme per 58000 MW protein. The major part of the enzyme is converted by the elution procedure to the inactive P420 form, a minor part is in its native heme-thiolate conformation; hemoprotein
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.01
-
prostaglandin H2
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.5
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.5
58000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.5
one-step immunoaffinity purification
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.5
platelet
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.5
0.255
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
Prostaglandin H2
i.e. PGH2
3194
Homo sapiens
Thromboxane B2 + 12(L)-hydroxy-5,8,10-heptadecatrienoic acid
the ratio of thromboxane to C17 hydroxyfatty acid formation is 1:1
3194
Homo sapiens
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.5
?
-
Homo sapiens
5.3.99.5
monomer
x * 58000, SDS-PAGE
Homo sapiens