EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.14 | Ca2+ | - |
Alcaligenes sp. | |
3.5.1.14 | Co2+ | - |
Alcaligenes sp. | |
3.5.1.14 | Cu2+ | - |
Alcaligenes sp. | |
3.5.1.14 | dithiothreitol | - |
Alcaligenes sp. | |
3.5.1.14 | EDTA | - |
Alcaligenes sp. | |
3.5.1.14 | Fe2+ | - |
Alcaligenes sp. | |
3.5.1.14 | Hg2+ | - |
Alcaligenes sp. | |
3.5.1.14 | iodoacetic acid | - |
Alcaligenes sp. | |
3.5.1.14 | Mn2+ | - |
Alcaligenes sp. | |
3.5.1.14 | N-ethylmaleimide | - |
Alcaligenes sp. | |
3.5.1.14 | Ni2+ | - |
Alcaligenes sp. | |
3.5.1.14 | p-chloromercuribenzoate | - |
Alcaligenes sp. | |
3.5.1.14 | Zn2+ | - |
Alcaligenes sp. | |
3.5.1.83 | CaCl2 | - |
Achromobacter xylosoxidans | |
3.5.1.83 | CoCl2 | - |
Achromobacter xylosoxidans | |
3.5.1.83 | Cu2+ | - |
Achromobacter xylosoxidans | |
3.5.1.83 | dithiothreitol | - |
Achromobacter xylosoxidans | |
3.5.1.83 | FeCl2 | - |
Achromobacter xylosoxidans | |
3.5.1.83 | Hg2+ | - |
Achromobacter xylosoxidans | |
3.5.1.83 | iodoacetic acid | - |
Achromobacter xylosoxidans | |
3.5.1.83 | MnCl2 | - |
Achromobacter xylosoxidans | |
3.5.1.83 | NEM | - |
Achromobacter xylosoxidans | |
3.5.1.83 | Ni2+ | - |
Achromobacter xylosoxidans | |
3.5.1.83 | PCMB | - |
Achromobacter xylosoxidans | |
3.5.1.83 | ZnCl2 | - |
Achromobacter xylosoxidans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.14 | 0.033 | - |
N-butyryl-D-aspartate | - |
Alcaligenes sp. | |
3.5.1.14 | 0.194 | - |
N-propionyl-D-aspartate | - |
Alcaligenes sp. | |
3.5.1.14 | 1.11 | - |
N-glycyl-D-aspartate | - |
Alcaligenes sp. | |
3.5.1.14 | 2.52 | - |
N-acetyl-D-aspartate | - |
Alcaligenes sp. | |
3.5.1.14 | 12.5 | - |
N-formyl-D-aspartate | - |
Alcaligenes sp. | |
3.5.1.83 | 0.033 | - |
N-Butanoyl-D-Asp | - |
Achromobacter xylosoxidans | |
3.5.1.83 | 0.194 | - |
N-Propanoyl-D-Asp | - |
Achromobacter xylosoxidans | |
3.5.1.83 | 1.11 | - |
N-Glycyl-D-Asp | - |
Achromobacter xylosoxidans | |
3.5.1.83 | 2.52 | - |
N-Acetyl-D-Asp | - |
Achromobacter xylosoxidans | |
3.5.1.83 | 12.5 | - |
N-Formyl-D-Asp | - |
Achromobacter xylosoxidans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 54000 | - |
1 * 54000, SDS-PAGE | Alcaligenes sp. |
3.5.1.14 | 56000 | - |
gel filtration | Alcaligenes sp. |
3.5.1.83 | 54000 | - |
1 * 54000, SDS-PAGE | Achromobacter xylosoxidans |
3.5.1.83 | 56000 | - |
gel filtration | Achromobacter xylosoxidans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.14 | N-acetyl-L-methionine | Alcaligenes sp. | - |
L-methionine + acetate | - |
? | |
3.5.1.83 | additional information | Achromobacter xylosoxidans | inducible by N-acetyl-D-Asp | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.14 | Alcaligenes sp. | - |
xylosoxydans subsp. xylosoxydans A-6 | - |
3.5.1.83 | Achromobacter xylosoxidans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.14 | - |
Alcaligenes sp. |
3.5.1.83 | - |
Achromobacter xylosoxidans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.83 | 175 | - |
- |
Achromobacter xylosoxidans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.14 | N-acetyl-D-aspartate + H2O | - |
Alcaligenes sp. | ? | - |
? | |
3.5.1.14 | N-acetyl-DL-aspartate + H2O | D-isomer, N-acetyl-D-aspartate as inducer | Alcaligenes sp. | acetate + DL-aspartate | - |
? | |
3.5.1.14 | N-acetyl-DL-methionine + H2O | - |
Alcaligenes sp. | DL-methionine + acetate | - |
? | |
3.5.1.14 | N-acetyl-L-methionine | - |
Alcaligenes sp. | L-methionine + acetate | - |
? | |
3.5.1.14 | N-butyryl-D-aspartate + H2O | - |
Alcaligenes sp. | butanoate + D-aspartate | - |
? | |
3.5.1.14 | N-formyl-D-aspartate + H2O | - |
Alcaligenes sp. | formate + D-aspartate | - |
? | |
3.5.1.14 | N-glycyl-D-aspartate + H2O | - |
Alcaligenes sp. | glycine + D-aspartate | - |
? | |
3.5.1.14 | N-propionyl-D-aspartate + H2O | - |
Alcaligenes sp. | proipionate + D-aspartate | - |
? | |
3.5.1.83 | Gly-D-Asp + H2O | - |
Achromobacter xylosoxidans | Glycine + Asp | - |
? | |
3.5.1.83 | additional information | no activity with N-carbobenzoyl-D-Asp, N-acetyl-L-Asp, N-acetyl-D-Glu | Achromobacter xylosoxidans | ? | - |
? | |
3.5.1.83 | additional information | inducible by N-acetyl-D-Asp | Achromobacter xylosoxidans | ? | - |
? | |
3.5.1.83 | N-Acetyl-D-Asp + H2O | - |
Achromobacter xylosoxidans | acetate + D-Asp | - |
? | |
3.5.1.83 | N-Butanoyl-D-Asp + H2O | - |
Achromobacter xylosoxidans | Butanoate + D-Asp | - |
? | |
3.5.1.83 | N-Formyl-D-Asp + H2O | - |
Achromobacter xylosoxidans | Formate + D-Asp | - |
? | |
3.5.1.83 | N-glycyl-D-Asp + H2O | - |
Achromobacter xylosoxidans | Gly + D-Asp | - |
? | |
3.5.1.83 | N-Propanoyl-D-Asp + H2O | - |
Achromobacter xylosoxidans | Propanoate + D-Asp | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.14 | monomer | 1 * 54000, SDS-PAGE | Alcaligenes sp. |
3.5.1.83 | monomer | 1 * 54000, SDS-PAGE | Achromobacter xylosoxidans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.14 | D-AAse | - |
Alcaligenes sp. |
3.5.1.14 | N-Acyl-D-aspartate amidohydrolase | - |
Alcaligenes sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 50 | - |
- |
Alcaligenes sp. |
3.5.1.83 | 50 | - |
- |
Achromobacter xylosoxidans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 25 | 75 | - |
Alcaligenes sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 45 | - |
thermostable up to, 60% activity remains at 50°C | Alcaligenes sp. |
3.5.1.83 | 45 | - |
10 min, stable up to | Achromobacter xylosoxidans |
3.5.1.83 | 50 | - |
10 min, 40% loss of activity | Achromobacter xylosoxidans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 7.5 | 8 | - |
Alcaligenes sp. |
3.5.1.83 | 7.5 | 8 | - |
Achromobacter xylosoxidans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 8 | - |
most stable in borate buffer | Alcaligenes sp. |
3.5.1.83 | 8 | - |
stable | Achromobacter xylosoxidans |