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Literature summary extracted from

  • Sugie, M.; Suzuki, H.
    Purification and properties of D-aminoacylase of Streptomyces olivaceus (1978), Agric. Biol. Chem., 42, 107-133.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.1.81 CoCl2 protects from denaturation Streptomyces olivaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.81 Ag+
-
 Streptomyces olivaceus
3.5.1.81 Cu2+
-
 Streptomyces olivaceus
3.5.1.81 EDTA
-
 Streptomyces olivaceus
3.5.1.81 Fe2+ weak Streptomyces olivaceus
3.5.1.81 Hg2+
-
 Streptomyces olivaceus
3.5.1.81 Ni2+ weak Streptomyces olivaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.81 1.13
-
 N-Acetyl-D-phenylglycine
-
 Streptomyces olivaceus
3.5.1.81 1.71
-
 N-Acetyl-D-Phe
-
 Streptomyces olivaceus
3.5.1.81 2.43
-
 N-Acetyl-D-Leu
-
 Streptomyces olivaceus
3.5.1.81 2.9
-
 N-Acetyl-D-Trp
-
 Streptomyces olivaceus
3.5.1.81 2.95
-
 N-Acetyl-D-Phe
-
 Streptomyces olivaceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.81 Co2+ activation Streptomyces olivaceus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Streptomyces olivaceus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.81 partial Streptomyces olivaceus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.81 60 min preincubation with 1 mM Na2EDTA at 30°C causes irreversible inactivation Streptomyces olivaceus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.81 571.1
-
-
 Streptomyces olivaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 N-acetyl-D-Leu + H2O
-
 Streptomyces olivaceus acetate + D-Leu
-
 ?
3.5.1.81 N-Acetyl-D-Phe + H2O
-
 Streptomyces olivaceus acetate + D-Phe
-
 ?
3.5.1.81 N-Acetyl-D-phenylglycine + H2O
-
 Streptomyces olivaceus acetate + D-phenylglycine
-
 ?
3.5.1.81 N-Acetyl-D-Trp + H2O
-
 Streptomyces olivaceus acetate + D-Trp
-
 ?
3.5.1.81 N-acetyl-D-Val + H2O
-
 Streptomyces olivaceus acetate + D-Val
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.81 50
-
-
 Streptomyces olivaceus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.81 30 60 30°C: about 50% of maximal activity, 60°C: about 30% of maximal activity Streptomyces olivaceus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.81 40
-
 pH 7.0, 15 min, stable up to Streptomyces olivaceus
3.5.1.81 60
-
 pH 7.0, 15 min, complete inactivation Streptomyces olivaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.81 7
-
-
 Streptomyces olivaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.81 6.3 9.3 pH 6.3: about 30% of maximal activity, pH 9.3: about 70% of maximal activity Streptomyces olivaceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.81 5
-
 5°C, 20 h, complete loss of activity Streptomyces olivaceus
3.5.1.81 5.5
-
 5°C, 20 h, about 50% loss of activity Streptomyces olivaceus
3.5.1.81 6
-
 5°C, 20 h, stable Streptomyces olivaceus
3.5.1.81 7.5
-
 5°C, 20 h, about 40% loss of activity Streptomyces olivaceus