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Literature summary extracted from

  • Louwrier, A.; Knowles, C.J.
    The purification and characterization of a novel D(-)-specific carbamoylase enzyme from an Agrobacterium sp. (1996), Enzyme Microb. Technol., 19, 562-571.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.77 Ba2+
-
 Agrobacterium sp.
3.5.1.77 Ca2+
-
 Agrobacterium sp.
3.5.1.77 Co2+
-
 Agrobacterium sp.
3.5.1.77 Cu2+
-
 Agrobacterium sp.
3.5.1.77 Fe2+
-
 Agrobacterium sp.
3.5.1.77 iodoacetamide
-
 Agrobacterium sp.
3.5.1.77 iodoacetate
-
 Agrobacterium sp.
3.5.1.77 K+
-
 Agrobacterium sp.
3.5.1.77 Li+
-
 Agrobacterium sp.
3.5.1.77 Mg2+ 100 nM Agrobacterium sp.
3.5.1.77 Na+
-
 Agrobacterium sp.
3.5.1.77 NEM
-
 Agrobacterium sp.
3.5.1.77 NH4+
-
 Agrobacterium sp.
3.5.1.77 Ni2+
-
 Agrobacterium sp.
3.5.1.77 Zn2+
-
 Agrobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.77 0.82
-
 N-carbamoyl-D-p-hydroxyphenylglycine
-
 Agrobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.77 additional information no metal ion requirement Agrobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.77 38100
-
 2 * 38100, SDS-PAGE Agrobacterium sp.
3.5.1.77 84000
-
 gel filtration Agrobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.77 Agrobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.77
-
 Agrobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.77 30
-
-
 Agrobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.77 additional information strictly D-specific Agrobacterium sp. ?
-
 ?
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium sp. D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-phenylglycine + H2O
-
 Agrobacterium sp. D-phenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Tyr + H2O
-
 Agrobacterium sp. D-Tyr + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Val + H2O
-
 Agrobacterium sp. D-Val + NH3 + CO2
-
 ?
3.5.1.77 N-Carbamoyl-DL-Met + H2O
-
 Agrobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.77 dimer 2 * 38100, SDS-PAGE Agrobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.77 70
-
-
 Agrobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.77 7.3 7.4
-
 Agrobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.77 6.2 8.7 pH 6.2: about 45% of maximal activity, pH 8.7: about 35% of maximal activity Agrobacterium sp.