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Literature summary extracted from

  • Nanba, H.; Ikenaka, Y.; Yamada, Y.; Yajima, K.; Takano, M.; Takahashi, S.
    Isolation of Agrobacterium sp. strain KNK712 that produces N-carbamyl-D-amino acid amidohydrolase, cloning of the gene for this enzyme, and properties of the enzyme (1998), Biosci. Biotechnol. Biochem., 62, 875-881.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.77 synthesis the enzyme is suitable for use in the production of D-amino acids Agrobacterium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.77 expression in Escherichia coli Agrobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.77 Ag+
-
 Agrobacterium sp.
3.5.1.77 Cu2+
-
 Agrobacterium sp.
3.5.1.77 Hg2+
-
 Agrobacterium sp.
3.5.1.77 NH4+
-
 Agrobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.77 34285
-
 2 * 34285, calculation from nucleotide sequence Agrobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.77 Agrobacterium sp.
-
-
-
3.5.1.77 Agrobacterium sp. KNK712
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.77
-
 Agrobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.77 N-carbamoyl-D-Ala + H2O 81% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Ala + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Ala + H2O 81% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. KNK712 D-Ala + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Asp + H2O 81% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Asp + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Ile + H2O 72% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Ile + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Leu + H2O as active as with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Leu + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Leu + H2O as active as with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. KNK712 D-Leu + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium sp. D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Phe + H2O 53% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Phe + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Phe + H2O 53% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. KNK712 D-Phe + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-phenylglycine + H2O 110% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-phenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Ser + H2O 55% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Ser + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Ser + H2O 55% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. KNK712 D-Ser + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Trp + H2O 42% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Trp + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Val + H2O 38% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. D-Val + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Val + H2O 38% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. KNK712 D-Val + NH3 + CO2
-
 ?
3.5.1.77 N-Carbamoyl-DL-2-amino-n-butyric acid + H2O 110% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-Met + H2O 160% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-norleucine + H2O 105% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-norvaline + H2O 76% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-Thr + H2O 27% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-Gly + H2O 12% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium sp. Gly + NH3 + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.77 dimer 2 * 34285, calculation from nucleotide sequence Agrobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.77 65
-
-
 Agrobacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.77 40 70 40°C: about 50% of maximal activity, 70°C: about 70% of maximal activity Agrobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.77 55
-
 pH 7.0, 10 min, stable Agrobacterium sp.
3.5.1.77 60
-
 pH 7.0, 10 min, about 25% loss of activity Agrobacterium sp.
3.5.1.77 65
-
 pH 7.0, 10 min, complete loss of activity Agrobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.77 7
-
-
 Agrobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.77 6 8.2 pH 6.0: about 50% of maximal activity, pH 8.2: about 65% loss of maximal activity Agrobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.77 7
-
 40°C, 5 h, stable Agrobacterium sp.