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Literature summary extracted from

  • Shimizu, S.; Ogawa, J.; Chung, M.C.M.; Yamada, H.
    Purification and characterization of a novel enzyme, arylalkyl acylamidase, from Pseudomonas putida Sc2 (1992), Eur. J. Biochem., 209, 375-382.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.76 1,10-phenanthroline
-
 Pseudomonas putida
3.5.1.76 2,2'-dipyridyl
-
 Pseudomonas putida
3.5.1.76 5,5'-dithiobis(2-nitrobenzoic acid) weak Pseudomonas putida
3.5.1.76 Ag2SO4
-
 Pseudomonas putida
3.5.1.76 HgCl2
-
 Pseudomonas putida
3.5.1.76 iodoacetate weak Pseudomonas putida
3.5.1.76 N-bromosuccinimide
-
 Pseudomonas putida
3.5.1.76 NEM
-
 Pseudomonas putida
3.5.1.76 PCMB
-
 Pseudomonas putida
3.5.1.76 phenylhydrazine
-
 Pseudomonas putida
3.5.1.76 Semicarbazide
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.76 0.31
-
 N-acetyl-2-phenylethylamine
-
 Pseudomonas putida
3.5.1.76 0.34
-
 N-acetylserotonin
-
 Pseudomonas putida
3.5.1.76 1.5
-
 Melatonin
-
 Pseudomonas putida
3.5.1.76 1.6
-
 N-Acetyl-3-phenylpropylamine
-
 Pseudomonas putida
3.5.1.76 5
-
 N-acetyldopamine
-
 Pseudomonas putida
3.5.1.76 8.9
-
 (R)-N-acetyl-1-methyl-3-phenylpropylamine
-
 Pseudomonas putida
3.5.1.76 41
-
 N-Acetylbenzylamine
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.76 extracellular
-
 Pseudomonas putida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.76 CdCl2 activates Pseudomonas putida
3.5.1.76 CoCl2 activates Pseudomonas putida
3.5.1.76 CuSO4 activates Pseudomonas putida
3.5.1.76 MgCl2 activates Pseudomonas putida
3.5.1.76 MnCl2 activates Pseudomonas putida
3.5.1.76 NiCl2 activates Pseudomonas putida
3.5.1.76 ZnSO4 activates Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.76 42000
-
 4 * 42000, SDS-PAGE Pseudomonas putida
3.5.1.76 42630
-
 4 * 42630, SDS-PAGE Pseudomonas putida
3.5.1.76 150000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.76 Pseudomonas putida
-
-
-
3.5.1.76 Pseudomonas putida Sc2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.76
-
 Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.76 culture medium
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.76 6.25
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.76 (R)-N-acetyl-1-methyl-3-phenylpropylamine + H2O
-
 Pseudomonas putida (S)-1-methyl-3-phenylpropylamine + acetate
-
 ?
3.5.1.76 Acetic acid 2-phenylethyl ester + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.1.76 Acetic acid 3-phenylpropyl ester + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.1.76 Melatonin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.1.76 additional information no activity with acetanilide derivatives Pseudomonas putida ?
-
 ?
3.5.1.76 additional information no activity with acetanilide derivatives Pseudomonas putida Sc2 ?
-
 ?
3.5.1.76 N-Acetyl-1-methyl-3-phenylpropylamine + H2O (R)-isomer Pseudomonas putida (S)-1-Methyl-3-phenylpropylamine + acetate
-
 ?
3.5.1.76 N-Acetyl-1-methyl-3-phenylpropylamine + H2O (R)-isomer Pseudomonas putida Sc2 (S)-1-Methyl-3-phenylpropylamine + acetate
-
 ?
3.5.1.76 N-Acetyl-2-phenylethylamine + H2O
-
 Pseudomonas putida 2-Phenylethylamine + acetate
-
 ?
3.5.1.76 N-Acetyl-2-phenylethylamine + H2O
-
 Pseudomonas putida Sc2 2-Phenylethylamine + acetate
-
 ?
3.5.1.76 N-Acetyl-3-phenylpropylamine
-
 Pseudomonas putida ?
-
 ?
3.5.1.76 N-Acetyl-3-phenylpropylamine
-
 Pseudomonas putida Sc2 ?
-
 ?
3.5.1.76 N-Acetylbenzylamine + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.1.76 N-Acetylbenzylamine + H2O
-
 Pseudomonas putida Sc2 ?
-
 ?
3.5.1.76 N-acetyldopamine + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.1.76 N-Acetylserotonin + H2O
-
 Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.76 tetramer 4 * 42000, SDS-PAGE Pseudomonas putida
3.5.1.76 tetramer 4 * 42630, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.76 70
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.76 55 75 55°C: about 45% of maximal activity, 75°C: about 20% of maximal activity Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.76 60
-
 pH 7.0, stable below Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.76 8
-
 with N-acetyl-2-phenylethylamine as substrate Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.76 6 10 pH 6.0: about 50% of maximal activity, pH 10: about 40% of maximal activity, with N-acetyl-2-phenylethalamine as substrate Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.76 7
-
 30°C, 30 min, most stable under alkaline conditions Pseudomonas putida