BRENDA - Enzyme Database show

Immunoaffinity purification and characterization of thromboxane synthase from porcine lung

Shen, R.F.; Tai, H.H.; J. Biol. Chem. 261, 11592-11599 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.5
(E)-3-[4-(1-Imidazolylmethyl)phenyl]-2-propenoate
reversible
Sus scrofa
5.3.99.5
15-hydroperoxyeicosatetraenoic acid
-
Sus scrofa
5.3.99.5
15-Hydroxy-11alpha,9alpha-(epoxymethano)-prosta-5,13-dienoic acid
reversible
Sus scrofa
5.3.99.5
15-Hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
irreversible inactivation during catalysis, protection by (E)-3-[4-(1-imidazolylmethyl)phenyl]-2-propenoate and 15-hydroxy-11alpha,9alpha-(epoxymethano)-prosta-5,13-dienoic acid
Sus scrofa
5.3.99.5
Cu2+
1 mM, complete inhibition
Sus scrofa
5.3.99.5
Zn2+
1 mM, 50% inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.012
-
15-hydroxy-9alpha,11alpha-peroxiprosta-5,13-dienoic acid
-
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.5
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.5
immunoaffinity purification
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.5
lung
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.5
0.231
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
Prostaglandin H2
i.e. PGH2
3166
Sus scrofa
Thromboxane B2 + 12(L)-hydroxy-5,8,10-heptadecatrienoic acid
the ratio of 12-hydroxy-5,8,10-heptadecatrienoic acid to thromboxane B2 varries from 1.6 to 2.1 depending on the reaction conditions
3166
Sus scrofa
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.99.5
30
-
-
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.5
heme
hemoprotein
Sus scrofa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.3.99.5
1
-
Zn2+
-
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.5
heme
hemoprotein
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.5
(E)-3-[4-(1-Imidazolylmethyl)phenyl]-2-propenoate
reversible
Sus scrofa
5.3.99.5
15-hydroperoxyeicosatetraenoic acid
-
Sus scrofa
5.3.99.5
15-Hydroxy-11alpha,9alpha-(epoxymethano)-prosta-5,13-dienoic acid
reversible
Sus scrofa
5.3.99.5
15-Hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid
irreversible inactivation during catalysis, protection by (E)-3-[4-(1-imidazolylmethyl)phenyl]-2-propenoate and 15-hydroxy-11alpha,9alpha-(epoxymethano)-prosta-5,13-dienoic acid
Sus scrofa
5.3.99.5
Cu2+
1 mM, complete inhibition
Sus scrofa
5.3.99.5
Zn2+
1 mM, 50% inhibition
Sus scrofa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.3.99.5
1
-
Zn2+
-
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.012
-
15-hydroxy-9alpha,11alpha-peroxiprosta-5,13-dienoic acid
-
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.5
immunoaffinity purification
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.5
lung
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.5
0.231
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
Prostaglandin H2
i.e. PGH2
3166
Sus scrofa
Thromboxane B2 + 12(L)-hydroxy-5,8,10-heptadecatrienoic acid
the ratio of 12-hydroxy-5,8,10-heptadecatrienoic acid to thromboxane B2 varries from 1.6 to 2.1 depending on the reaction conditions
3166
Sus scrofa
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.99.5
30
-
-
Sus scrofa