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Literature summary extracted from

  • Obisco, R.J.; Azghani, A.O.; Wilkins, T.D.
    The Bacteroides fragilis toxin fragilysin disrupts the paracellular barrier of epitheliar cells (1997), Infect. Immun., 65, 1431-1439.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.74 1,10-phenanthroline
-
 Bacteroides fragilis
3.4.24.74 EDTA
-
 Bacteroides fragilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.74 extracellular
-
 Bacteroides fragilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.74 20600
-
 1 * 20600, SDS-PAGE Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.74 Proteins + H2O Bacteroides fragilis the enzyme increases the paracellular permeability of the intestinal epithelium by digestion of the intestinal light junctions and cell-to-cell contacts, it does not disrupt cell membranes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.74 Bacteroides fragilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.74
-
 Bacteroides fragilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.74 culture filtrate
-
 Bacteroides fragilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.74 additional information
-
 specific activity is expressed as a cytotoxic effect in cell-rounding titers of Ht-29 cells (i.e. colon carcinoma cells) Bacteroides fragilis

Storage Stability

EC Number Storage Stability Organism
3.4.24.74 -30°C Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.74 Proteins + H2O the enzyme increases the paracellular permeability of the intestinal epithelium by digestion of the intestinal light junctions and cell-to-cell contacts, it does not disrupt cell membranes Bacteroides fragilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.74 monomer 1 * 20600, SDS-PAGE Bacteroides fragilis