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Literature summary extracted from

  • Takahashi, M.; Matsushita, Y.; Iijima, Y.; Tanzawa, K.
    Purification and characterization of endothelin-converting enzyme from rat lung (1993), J. Biol. Chem., 268, 21394-21398.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.71 1,10-phenanthroline
-
 Rattus norvegicus
3.4.24.71 EDTA
-
 Rattus norvegicus
3.4.24.71 additional information no inhibition by thiorphan, captopril Rattus norvegicus
3.4.24.71 phosphoramidon
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.71 0.0002
-
 Big endothelin-1
-
 Rattus norvegicus
3.4.24.71 0.00027
-
 big endothelin-3
-
 Rattus norvegicus
3.4.24.71 0.00046
-
 big endothelin-2
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.71 membrane
-
 Rattus norvegicus 16020
-
3.4.24.71 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.71 additional information neutral metalloendopeptidase Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.71 130000
-
 1 * 130000, SDS-PAGE Rattus norvegicus
3.4.24.71 170000 190000 rat, FPLC gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.71 Big endothelin 1 + H2O Rattus norvegicus key enzyme in endothelin production, generates potent vasoconstrictor endothelin from its inactive precursor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.71 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.71
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.71 lung lung enzyme is similar to that of endothelial cells Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.71 0.00127
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.71 Big endothelin 1 + H2O key enzyme in endothelin production, generates potent vasoconstrictor endothelin from its inactive precursor Rattus norvegicus ?
-
 ?
3.4.24.71 big endothelin-2 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.71 big endothelin-3 + H2O
-
 Rattus norvegicus endothelin-3 + ?
-
 ?
3.4.24.71 Human big endothelin 1 + H2O best substrate Rattus norvegicus Endothelin 1 + big endothelin 1(21-38)
-
 ?
3.4.24.71 Human big endothelin 2 + H2O hydrolysis at 25% the rate of big endothelin1 Rattus norvegicus ?
-
 ?
3.4.24.71 Human big endothelin 3 + H2O hydrolysis at 50% the rate of big endothelin1 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.71 monomer 1 * 130000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.71 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.71 6.6 6.8
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.71 6.5 7.2 active in a narrow range Rattus norvegicus