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Literature summary extracted from

  • Schiavo, G.; Shone, C.C.; Rossetto, O.; Alexander, F.C.G.; Montecucco, C.
    Botulinum neurotoxin serotype F is a zinc endopeptidase specific for VAMP/synaptobrevin (1993), J. Biol. Chem., 268, 11516-11519.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.69 1,10-phenanthroline r, Zn2+ restores Clostridium sp.
3.4.24.69 captopril
-
 Clostridium sp.
3.4.24.69 EDTA r, Zn2+ restores Clostridium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.69 additional information no involvement of cobalt, copper, iron, manganese or nickel, atomic absorption spectroscopy Clostridium sp.
3.4.24.69 Zinc zinc-dependent endopeptidase Clostridium sp.
3.4.24.69 Zinc atom absorption spectroscopy Clostridium sp.
3.4.24.69 Zinc 1 atom of zinc per molecule botulinum neurotoxin, bound to light chain (i.e. L-chain) Clostridium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.69 additional information
-
 amino acid sequence similarity of clostridial neurotoxins Clostridium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.69 Clostridium sp.
-
 F
-
3.4.24.69 Clostridium sp. F
-
 F
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.69 Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O serotype BoNT/F: cleavage at Gln-Lys Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O both isoforms are cleaved at the same rate Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O highly specific neurotoxins Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium sp. F Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O serotype BoNT/F: cleavage at Gln-Lys Clostridium sp. F Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O both isoforms are cleaved at the same rate Clostridium sp. F Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O highly specific neurotoxins Clostridium sp. F Hydrolyzed synaptobrevin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.69 37
-
 assay at Clostridium sp.